The product has been successfully added to your shopping list.

4-Nitrophenyl-α-D-glucopyranoside

4-Nitrophenyl-alpha-D-glucopyranoside O-PNPAG
Product code: O-PNPAG
€96.00

2 g

Prices exclude VAT

Available for shipping

North American customers click here
Content: 2 g
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Physical Form: Powder
Stability: > 10 years under recommended storage conditions
CAS Number: 3767-28-0
Synonyms: p-Nitrophenyl-α-D-glucopyranoside, pNP-α-D-glucopyranoside
Molecular Formula: C12H15NO8
Molecular Weight: 301.3
Purity: > 98%
Substrate For (Enzyme): α-Glucosidase
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400-420

High purity 4-Nitrophenyl-α-D-glucopyranoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for the measurement of α-glucosidase activity.

See our full list of colourimetric oligosaccharides.

Documents
Certificate of Analysis
Safety Data Sheet
Data Sheet
Publications
Megazyme publication

Diastatic power and maltose value: a method for the measurement of amylolytic enzymes in malt.

Charmier, L. M., McLoughlin, C. & McCleary, B. V. (2021). Journal of the Institute of Brewing, In Press.

A simple method for measurement of the amylolytic activity of malt has been developed and fully evaluated. The method, termed the Maltose Value (MV) is an extension of previously reported work. Here, the MV method has been studied in detail and all aspects of the assay (sample grinding and extraction, starch hydrolysis, maltose hydrolysis and determination as glucose) have been optimised. The method is highly correlated with other dextrinising power methods. The MV method involves extraction of malt in 0.5% sodium chloride at 30°C for 20 minutes followed by filtration; incubation of an aliquot of the undiluted filtrate with starch solution (pH 4.6) at 30°C for 15 min; termination of reaction with sodium hydroxide solution; dilution of sample in an appropriate buffer; hydrolysis of maltose with a specific α-glucosidase; glucose determination and activity calculation. Unlike all subsequent reducing sugar methods, the maltose value method measures a defined reaction product, maltose, with no requirement to use equations to relate analytical values back to Lintner units. The maltose value method is the first viable method in 130 years that could effectively replace the 1886 Lintner method.

Hide Abstract
Safety Information
Symbol : GHS07
Signal Word : Warning
Hazard Statements : H315, H319, H335
Precautionary Statements : P261, P264, P271, P280, P302+P352, P304+P340
Safety Data Sheet
Customers also viewed
4-Nitrophenyl-alpha-D-mannopyranoside O-PNPAM
4-Nitrophenyl-α-D-mannopyranoside
€106.00
alpha-Glucosidase Aspergillus niger E-TRNGL
α-Glucosidase (Aspergillus niger)
€142.00
alpha-Glucosidase yeast maltase E-MALTS
α-Glucosidase (yeast maltase)
€156.00