Validated Methods
Most of the methods from Neogen®'s Megazyme range have been conferred validation by various international standard organisations, as listed below.
In addition to these formally recognised methods, a large number of our assay kits are based on principles recognised by national and regional standards authorities (such as EU Norms) or major industry bodies, e.g. the International Dairy Federation and MEBAK. All relevant accreditations can be found on the individual product pages.
- AOAC Method 985.09: Fructose/Glucose in Foods
D-Fructose/D-Glucose Assay Kit (K-FRUGL)
- AOAC Method 985.29: Total Dietary Fiber in Foods
Total Dietary Fiber Assay Kit (K-TDFR)
- AOAC Method 991.42:Insoluble Dietary Fiber in Foods and Food Products
Total Dietary Fiber Assay Kit (K-TDFR)
- AOAC Method 991.43: Total, Soluble, and Insoluble Dietary Fiber in Foods
Total Dietary Fiber Assay Kit (K-TDFR)
- AOAC Method 992.28: β-Glucan (mixed linkage) in Food and Food Products
β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- AOAC Method 993.19: Soluble Dietary Fiber in Food and Food Products
Total Dietary Fiber Assay Kit (K-TDFR)
- AOAC Method 995.16:β-D-Glucan in Oats
β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- AOAC Method 996.11:Starch (Total) in Cereal Products (Megazyme Amyloglucosidase/α-Amylase Method)
Total Starch Assay Kit (K-TSTA)
- AOAC Method 997.08: Total Fructan in Foods (Ion Exchange Chromatographic Method)
Amyloglucosidase (Aspergillus niger) (E-AMGFR), Fructanase Mixture (powder) (E-FRMXPD), Fructanase Mixture (liquid) (E-FRMXLQ)
- AOAC Method 999.03:Total Fructan in Foods (Enzymatic/Spectrophotometric Method)
Fructan Assay Kit (K-FRUC)
- AOAC Method 2000.11:Polydextrose in Foods
Fructanase Mixture (powder) (E-FRMXPD), Fructanase Mixture (liquid) (E-FRMXLQ), Amyloglucosidase (Aspergillus niger) (E-AMGDF), Amyloglucosidase (Aspergillus niger) Powder (E-AMGDFPD), Isoamylase (Glycogen 6-glucanohydrolase) (E-ISAMY), Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) (E-ISAMYFO)
- AOAC Method 2001.03:Dietary Fiber Containing Supplemented Resistant Maltodextrin (RMD)
Amyloglucosidase (Aspergillus niger) Powder (E-AMGDFPD)
- AOAC Method 2002.01:α-Amylase Activity in White Wheat Flour, Milled Malt and Microbial Enzyme Preparations
α-Amylase Assay Kit (Ceralpha Method) (K-CERA), Malt Amylase Assay Kit (K-MALTA)
- AOAC Method 2002.02:Resistant Starch in Starch and Plant Materials
Resistant Starch Assay Kit (K-RSTAR)
- AOAC Method 2006.06: Lactose in Milk (Enzymatic/Spectrophotometric Method)
Lactose/Galactose Assay Kit (K-LACGAR)
- AOAC Method 2009.01
Integrated Total Dietary Fiber Assay Kit (K-INTDF)
- AOAC Method 2011.25
Integrated Total Dietary Fiber Assay Kit (K-INTDF)
- AOAC Method 2016.06:Fructans in Infant, Adult and Pediatric Nutritional Formulas (HPAEC-PAD Method)
Amyloglucosidase (aspergillus niger) Powder (E-AMGDFPD), Fructanase Mixture (liquid) (E-FRMXLQ), Fructanase Mixture (powder) (E-FRMXPD), Sucrase (from yeast) (E-SUCR)
- AOAC Method 2016.14: Fructans in Infant Formula and Adult Nutrition (HPAEC-PAD Method)
Fructan Assay Kit (K-FRUC)
- AOAC Method 2017.07
Ethanol Assay Kit (Liquid Ready) (K-ETOHLQR)
- AOAC Method 2017.16: Total Dietary Fiber in Foods
Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)
- AOAC Method 2018.07
Fructan Assay Kit (K-FRUC)
- AOAC Method 2019.08
Ethanol Assay Kit (K-ETOH)
- AOAC Method 2020.07
Available Carbohydrates Assay Kit (K-AVCHO)
- AOAC Method 2020.08
Lactose Assay Kit (K-LOLAC)
- AOAC Method 2022.01
Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)
- AACC Method 22-02.01: Measurement of α-Amylase in Plant and Microbial Materials
α-Amylase Assay Kit (Ceralpha Method) (K-CERA), Malt Amylase Assay Kit (K-MALTA)
- AACC Method 22-05.01: Measurement of α-Amylase in Cereal Grains and Flours
Amylazyme (α-Amylase Assay) Tablets (T-AMZ)
- AACC Method 32-05.01: Total Dietary Fiber
Total Dietary Fiber Assay Kit (K-TDFR)
- AACC Method 32-06.01:Total Dietary Fiber - Rapid Gravimetric Method
Total Dietary Fiber Assay Kit (K-TDFR)
- AACC Method 32-07.01:Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products
Total Dietary Fiber Assay Kit (K-TDFR)
- AACC Method 32-21.01: Insoluble and Soluble Dietary Fiber in Oat Products - Enzymatic-Gravimetric Method
Total Dietary Fiber Assay Kit (K-TDFR)
- AACC Method 32-23.01: β-Glucan Content of Barley and Oats
β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- AACC Method 32-32.01:Measurement of Total Fructan in Foods by an Enzymatic/Spectrophotometric Method
Fructan Assay Kit (K-FRUC)
- AACC Method 32-40.01: Resistant Starch in Starch Samples and Plant Materials
Resistant Starch Assay Kit (K-RSTAR)
- AACC Method 32-45.01: Total Dietary Fiber (Codex Alimentarius Definition)
Integrated Total Dietary Fiber Assay Kit (K-INTDF)
- AACC Method 32-50.01: Insoluble, Soluble, and Total Dietary Fiber (Codex Definition) by an Enzymatic-Gravimetric Method and Liquid Chromatography
Integrated Total Dietary Fiber Assay Kit (K-INTDF)
- AACC Method 32-60.01: Rapid Integrated Method for the measurement of Total Dietary Fiber
Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)
- AACC Method 76-13.01: Total Starch Assay Procedure (Megazyme Amyloglucosidase/α-Amylase Method)
Total Starch Assay Kit (K-TSTA)
- AACC Method 76-31.01:Determination of Damaged Starch Spectrophotometric Method
Starch Damage Assay Kit (K-SDAM)
- Standard No. 164: Measurement of Damaged Starch
Starch Damage Assay Kit (K-SDAM)
- Standard No. 166: Measurement of β-Glucan (Mixed Linkage)
β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- Standard No. 168: Measurement of Total Starch Content of Cereal Grains, Food and Feed Products using Megazyme’s Amyloglucosidase/α-Amylase Method
Total Starch Assay Kit (K-TSTA)
- Standard No. 185: Measurement of Total Dietary Fiber in Cereals, Ingredients and Food Products Using the Rapid Integrated TDF Procedure (RINTDF)
Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)
- GB Standard 5009.88-2014
Total Dietary Fiber Assay Kit (K-TDFR)
- GB Standard 5009.245-2016
Amyloglucosidase (Aspergillus niger) (E-AMGDF)
Amyloglucosidase (Aspergillus niger) (E-AMGFR)
Fructanase Mixture (liquid) (E-FRMXLQ)
Fructanase Mixture (powder) (E-FRMXPD)
Isoamylase (Glycogen 6-glucanohydrolase) (E-ISAMY)
Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) (E-ISAMYFO)
- EBC Method 3.10.1
β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- EBC Method 3.10.2
β-Glucan CFA Standard (P-BGCFA)
- EBC Method 4.13
β-Amylase (Barley) (E-BARBL)
β-Amylase (Barley) Powder (E-BARBP)
Malt Amylase Standard (E-MAST)
β-Limit Dextrin (P-BLDX)
- EBC Method 4.16.2
β-Glucan CFA Standard (P-BGCFA)
- EBC Method 6.5
α-Amylase (Bacillus licheniformis) (E-BLAAM)
- EBC Method 8.13.2
β-Glucan CFA Standard (P-BGCFA)
- EBC Method 8.13.3
β-Glucan (Barley; Medium Viscosity) (P-BGBM)
- EBC Method 9.3.1
Ethanol Assay Kit (K-ETOH)
- EBC Method 9.31.2
β-Glucan CFA Standard (P-BGCFA)
- α-Amylase Assay Kit (Ceralpha Method) (K-CERA)
- β-Amylase Assay Kit (Betamyl-3) (K-BETA3)
- β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- β-Glucanase Assay Kit (Malt and Microbial) (K-MBGL)
- Malt Amylase Assay Kit (K-MALTA)
- Starch Damage Assay Kit (K-SDAM)
- Total Starch Assay Kit (AA/AMG) (K-TSTA)
- Amylazyme Tablets (T-AMZ)
- Limit-Dextrizyme Tablets (T-LDZ)
- Xylazyme AX Tablets (T-XAX)
- AOAC Method 985.09: Fructose/Glucose in Foods
D-Fructose/D-Glucose Assay Kit (K-FRUGL)
- AOAC Method 985.29: Total Dietary Fiber in Foods
Total Dietary Fiber Assay Kit (K-TDFR)
- AOAC Method 991.42:Insoluble Dietary Fiber in Foods and Food Products
Total Dietary Fiber Assay Kit (K-TDFR)
- AOAC Method 991.43: Total, Soluble, and Insoluble Dietary Fiber in Foods
Total Dietary Fiber Assay Kit (K-TDFR)
- AOAC Method 992.28: β-Glucan (mixed linkage) in Food and Food Products
β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- AOAC Method 993.19: Soluble Dietary Fiber in Food and Food Products
Total Dietary Fiber Assay Kit (K-TDFR)
- AOAC Method 995.16:β-D-Glucan in Oats
β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- AOAC Method 996.11:Starch (Total) in Cereal Products (Megazyme Amyloglucosidase/α-Amylase Method)
Total Starch Assay Kit (K-TSTA)
- AOAC Method 997.08: Total Fructan in Foods (Ion Exchange Chromatographic Method)
Amyloglucosidase (Aspergillus niger) (E-AMGFR), Fructanase Mixture (powder) (E-FRMXPD), Fructanase Mixture (liquid) (E-FRMXLQ)
- AOAC Method 999.03:Total Fructan in Foods (Enzymatic/Spectrophotometric Method)
Fructan Assay Kit (K-FRUC)
- AOAC Method 2000.11:Polydextrose in Foods
Fructanase Mixture (powder) (E-FRMXPD), Fructanase Mixture (liquid) (E-FRMXLQ), Amyloglucosidase (Aspergillus niger) (E-AMGDF), Amyloglucosidase (Aspergillus niger) Powder (E-AMGDFPD), Isoamylase (Glycogen 6-glucanohydrolase) (E-ISAMY), Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) (E-ISAMYFO)
- AOAC Method 2001.03:Dietary Fiber Containing Supplemented Resistant Maltodextrin (RMD)
Amyloglucosidase (Aspergillus niger) Powder (E-AMGDFPD)
- AOAC Method 2002.01:α-Amylase Activity in White Wheat Flour, Milled Malt and Microbial Enzyme Preparations
α-Amylase Assay Kit (Ceralpha Method) (K-CERA), Malt Amylase Assay Kit (K-MALTA)
- AOAC Method 2002.02:Resistant Starch in Starch and Plant Materials
Resistant Starch Assay Kit (K-RSTAR)
- AOAC Method 2006.06: Lactose in Milk (Enzymatic/Spectrophotometric Method)
Lactose/Galactose Assay Kit (K-LACGAR)
- AOAC Method 2009.01
Integrated Total Dietary Fiber Assay Kit (K-INTDF)
- AOAC Method 2011.25
Integrated Total Dietary Fiber Assay Kit (K-INTDF)
- AOAC Method 2016.06:Fructans in Infant, Adult and Pediatric Nutritional Formulas (HPAEC-PAD Method)
Amyloglucosidase (aspergillus niger) Powder (E-AMGDFPD), Fructanase Mixture (liquid) (E-FRMXLQ), Fructanase Mixture (powder) (E-FRMXPD), Sucrase (from yeast) (E-SUCR)
- AOAC Method 2016.14: Fructans in Infant Formula and Adult Nutrition (HPAEC-PAD Method)
Fructan Assay Kit (K-FRUC)
- AOAC Method 2017.07
Ethanol Assay Kit (Liquid Ready) (K-ETOHLQR)
- AOAC Method 2017.16: Total Dietary Fiber in Foods
Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)
- AOAC Method 2018.07
Fructan Assay Kit (K-FRUC)
- AOAC Method 2019.08
Ethanol Assay Kit (K-ETOH)
- AOAC Method 2020.07
Available Carbohydrates Assay Kit (K-AVCHO)
- AOAC Method 2020.08
Lactose Assay Kit (K-LOLAC)
- AOAC Method 2022.01
Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)
- AACC Method 22-02.01: Measurement of α-Amylase in Plant and Microbial Materials
α-Amylase Assay Kit (Ceralpha Method) (K-CERA), Malt Amylase Assay Kit (K-MALTA)
- AACC Method 22-05.01: Measurement of α-Amylase in Cereal Grains and Flours
Amylazyme (α-Amylase Assay) Tablets (T-AMZ)
- AACC Method 32-05.01: Total Dietary Fiber
Total Dietary Fiber Assay Kit (K-TDFR)
- AACC Method 32-06.01:Total Dietary Fiber - Rapid Gravimetric Method
Total Dietary Fiber Assay Kit (K-TDFR)
- AACC Method 32-07.01:Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products
Total Dietary Fiber Assay Kit (K-TDFR)
- AACC Method 32-21.01: Insoluble and Soluble Dietary Fiber in Oat Products - Enzymatic-Gravimetric Method
Total Dietary Fiber Assay Kit (K-TDFR)
- AACC Method 32-23.01: β-Glucan Content of Barley and Oats
β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- AACC Method 32-32.01:Measurement of Total Fructan in Foods by an Enzymatic/Spectrophotometric Method
Fructan Assay Kit (K-FRUC)
- AACC Method 32-40.01: Resistant Starch in Starch Samples and Plant Materials
Resistant Starch Assay Kit (K-RSTAR)
- AACC Method 32-45.01: Total Dietary Fiber (Codex Alimentarius Definition)
Integrated Total Dietary Fiber Assay Kit (K-INTDF)
- AACC Method 32-50.01: Insoluble, Soluble, and Total Dietary Fiber (Codex Definition) by an Enzymatic-Gravimetric Method and Liquid Chromatography
Integrated Total Dietary Fiber Assay Kit (K-INTDF)
- AACC Method 32-60.01: Rapid Integrated Method for the measurement of Total Dietary Fiber
Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)
- AACC Method 76-13.01: Total Starch Assay Procedure (Megazyme Amyloglucosidase/α-Amylase Method)
Total Starch Assay Kit (K-TSTA)
- AACC Method 76-31.01:Determination of Damaged Starch Spectrophotometric Method
Starch Damage Assay Kit (K-SDAM)
- Standard No. 164: Measurement of Damaged Starch
Starch Damage Assay Kit (K-SDAM)
- Standard No. 166: Measurement of β-Glucan (Mixed Linkage)
β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- Standard No. 168: Measurement of Total Starch Content of Cereal Grains, Food and Feed Products using Megazyme’s Amyloglucosidase/α-Amylase Method
Total Starch Assay Kit (K-TSTA)
- Standard No. 185: Measurement of Total Dietary Fiber in Cereals, Ingredients and Food Products Using the Rapid Integrated TDF Procedure (RINTDF)
Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)
- GB Standard 5009.88-2014
Total Dietary Fiber Assay Kit (K-TDFR)
- GB Standard 5009.245-2016
Amyloglucosidase (Aspergillus niger) (E-AMGDF)
Amyloglucosidase (Aspergillus niger) (E-AMGFR)
Fructanase Mixture (liquid) (E-FRMXLQ)
Fructanase Mixture (powder) (E-FRMXPD)
Isoamylase (Glycogen 6-glucanohydrolase) (E-ISAMY)
Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) (E-ISAMYFO)
- EBC Method 3.10.1
β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- EBC Method 3.10.2
β-Glucan CFA Standard (P-BGCFA)
- EBC Method 4.13
β-Amylase (Barley) (E-BARBL)
β-Amylase (Barley) Powder (E-BARBP)
Malt Amylase Standard (E-MAST)
β-Limit Dextrin (P-BLDX)
- EBC Method 4.16.2
β-Glucan CFA Standard (P-BGCFA)
- EBC Method 6.5
α-Amylase (Bacillus licheniformis) (E-BLAAM)
- EBC Method 8.13.2
β-Glucan CFA Standard (P-BGCFA)
- EBC Method 8.13.3
β-Glucan (Barley; Medium Viscosity) (P-BGBM)
- EBC Method 9.3.1
Ethanol Assay Kit (K-ETOH)
- EBC Method 9.31.2
β-Glucan CFA Standard (P-BGCFA)
- α-Amylase Assay Kit (Ceralpha Method) (K-CERA)
- β-Amylase Assay Kit (Betamyl-3) (K-BETA3)
- β-Glucan Assay Kit (Mixed Linkage) (K-BGLU)
- β-Glucanase Assay Kit (Malt and Microbial) (K-MBGL)
- Malt Amylase Assay Kit (K-MALTA)
- Starch Damage Assay Kit (K-SDAM)
- Total Starch Assay Kit (AA/AMG) (K-TSTA)
- Amylazyme Tablets (T-AMZ)
- Limit-Dextrizyme Tablets (T-LDZ)
- Xylazyme AX Tablets (T-XAX)
