|Formulation:||In 3.2 M ammonium sulphate|
|Stability:||Minimum 1 year at 4oC. Check vial for details.|
|Synonyms:||arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase|
|Concentration:||Supplied at ~ 750 U/mL|
|Expression:||Recombinant from Cellvibrio japonicus|
|Specificity:||endo-hydrolysis of (1,4)-β-D-galactosidic linkages in galactans.|
|Specific Activity:||~ 100 U/mg (40oC, pH 8.0 on potato galactan)|
|Unit Definition:||One Unit of galactanase activity is defined as the amount of enzyme required to release one µmole of galactose reducing-sugar equivalents per minute from potato galactan in sodium phosphate buffer (100 mM), pH 8.0 at 40oC.|
|Application examples:||Applications established in diagnostics and research within the food and feed, carbohydrate and biofuels industries.|
High purity recombinant endo-1,4-β-Galactanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Guan, J. & Li, S. P. (2010). Journal of Pharmaceutical and Biomedical Analysis, 51(3), 590-598.
Polysaccharides isolated from traditional Chinese medicines (TCMs) exhibit multiple pharmacological activities. However, quality control of polysaccharides is a challenge because of their complicate structure and macro-molecular mass. In this study, saccharide mapping based on specific enzymatic digestion of polysaccharides and chromatographic analysis was proposed to discriminate the polysaccharides from different TCMs. Endo-carbohydrase such as glucanase, arabinanase, xylanase, galactanase, cellulase, amylase and pectinase were used for enzymatic digestion of polysaccharides from 9 TCMs namely Panax ginseng, P. notoginseng, P. quinquefolium, Cordyceps sinensis, C. militaris, Ganoderma lucidum, G. sinense, Astragalus membranaceus and Angelica sinensis. By using high performance size exclusion chromatography (HPSEC) as well as derivatization with 1-Phenyl-3-methyl-5-pyrazolone (PMP) and HPLC analysis, the enzymatic hydrolysis properties of polysaccharides and their saccharide mapping were determined. The polysaccharides from 9 TCMs were firstly successfully distinguished based on their characteristic saccharide maps, which is helpful to improve the quality control of polysaccharides.Hide Abstract