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endo-1,4-β-Galactanase (Cellvibrio japonicus)

endo-1-4-beta-Galactanase Cellvibrio japonicus E-GALCJ
Product code: E-GALCJ
€173.00

1,500 Units

Prices exclude VAT

Available for shipping

Content: 1,500 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: endo-1,4-β-Galactanase
EC Number: 3.2.1.89
CAZy Family: GH53
CAS Number: 58182-40-4
Synonyms: arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase
Source: Cellvibrio japonicus
Molecular Weight: 41,100
Concentration: Supplied at ~ 750 U/mL
Expression: Recombinant from Cellvibrio japonicus
Specificity: endo-hydrolysis of (1,4)-β-D-galactosidic linkages in galactans.
Specific Activity: ~ 100 U/mg (40oC, pH 8.0 on potato galactan)
Unit Definition: One Unit of galactanase activity is defined as the amount of enzyme required to release one µmole of galactose reducing-sugar equivalents per minute from potato galactan in sodium phosphate buffer (100 mM), pH 8.0 at 40oC.
Temperature Optima: 40oC
pH Optima: 8
Application examples: Applications established in diagnostics and research within the food and feed, carbohydrate and biofuels industries.

High purity recombinant endo-1,4-β-Galactanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Publication
Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping—Enzymatic digestion followed by chromatographic analysis.

Guan, J. & Li, S. P. (2010). Journal of Pharmaceutical and Biomedical Analysis, 51(3), 590-598.

Polysaccharides isolated from traditional Chinese medicines (TCMs) exhibit multiple pharmacological activities. However, quality control of polysaccharides is a challenge because of their complicate structure and macro-molecular mass. In this study, saccharide mapping based on specific enzymatic digestion of polysaccharides and chromatographic analysis was proposed to discriminate the polysaccharides from different TCMs. Endo-carbohydrase such as glucanase, arabinanase, xylanase, galactanase, cellulase, amylase and pectinase were used for enzymatic digestion of polysaccharides from 9 TCMs namely Panax ginseng, P. notoginseng, P. quinquefolium, Cordyceps sinensis, C. militaris, Ganoderma lucidum, G. sinense, Astragalus membranaceus and Angelica sinensis. By using high performance size exclusion chromatography (HPSEC) as well as derivatization with 1-Phenyl-3-methyl-5-pyrazolone (PMP) and HPLC analysis, the enzymatic hydrolysis properties of polysaccharides and their saccharide mapping were determined. The polysaccharides from 9 TCMs were firstly successfully distinguished based on their characteristic saccharide maps, which is helpful to improve the quality control of polysaccharides.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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