endo-1,4-β-Galactanase (Cellvibrio japonicus)

Reference code: E-GALCJ

1,500 Units

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

1,500 Units

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

Minimum 1 year at 4^oC. Check vial for details.

Enzyme Activity:

endo-1,4-β-Galactanase

EC Number:

3.2.1.89

CAZy Family:

GH53

CAS Number:

58182-40-4

Synonyms:

arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase

Source:

Cellvibrio japonicus

Molecular Weight:

41,100

Concentration:

Supplied at ~ 750 U/mL

Expression:

Recombinant from Cellvibrio japonicus

Specificity:

endo-hydrolysis of (1,4)-β-D-galactosidic linkages in galactans.

Specific Activity:

~ 100 U/mg (40^oC, pH 8.0 on potato galactan)

Unit Definition:

One Unit of galactanase activity is defined as the amount of enzyme required to release one µmole of galactose reducing-sugar equivalents per minute from potato galactan in sodium phosphate buffer (100 mM), pH 8.0 at 40^oC.

Temperature Optima:

40^oC

pH Optima:

8

Application examples:

Applications established in diagnostics and research within the food and feed, carbohydrate and biofuels industries.

This product has been discontinued (read more).

High purity recombinant endo-1,4-β-Galactanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Explore all our available CAZy enzyme products.

Related Products

Related Carbohydrates

Galactan (Potato)

700005011 / P-GALPOT

Associated Enzymes

β-Galactosidase (Aspergillus niger)

700004192 / E-BGLAN

Publications

Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping—Enzymatic digestion followed by chromatographic analysis.

Guan, J. & Li, S. P. (2010). Journal of Pharmaceutical and Biomedical Analysis, 51(3), 590-598.

Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping—Enzymatic digestion followed by chromatographic analysis.

Guan, J. & Li, S. P. (2010). Journal of Pharmaceutical and Biomedical Analysis, 51(3), 590-598.

Polysaccharides isolated from traditional Chinese medicines (TCMs) exhibit multiple pharmacological activities. However, quality control of polysaccharides is a challenge because of their complicate structure and macro-molecular mass. In this study, saccharide mapping based on specific enzymatic digestion of polysaccharides and chromatographic analysis was proposed to discriminate the polysaccharides from different TCMs. Endo-carbohydrase such as glucanase, arabinanase, xylanase, galactanase, cellulase, amylase and pectinase were used for enzymatic digestion of polysaccharides from 9 TCMs namely Panax ginseng, P. notoginseng, P. quinquefolium, Cordyceps sinensis, C. militaris, Ganoderma lucidum, G. sinense, Astragalus membranaceus and Angelica sinensis. By using high performance size exclusion chromatography (HPSEC) as well as derivatization with 1-Phenyl-3-methyl-5-pyrazolone (PMP) and HPLC analysis, the enzymatic hydrolysis properties of polysaccharides and their saccharide mapping were determined. The polysaccharides from 9 TCMs were firstly successfully distinguished based on their characteristic saccharide maps, which is helpful to improve the quality control of polysaccharides.