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|Storage Temperature:||Below -10oC|
|Formulation:||In 50% (v/v) glycerol|
|Stability:||Minimum 1 year at < -10oC. Check vial for details.|
|Synonyms:||glucan endo-1,3-beta-D-glucosidase; 3-beta-D-glucan glucanohydrolase|
|Concentration:||Supplied at ~ 2,100 U/mL|
|Expression:||Recombinant from Hordeum vulgare|
|Specificity:||endo-hydrolysis of (1,3)-β-D-glucosidic linkages in (1,3)-β-D-glucans.|
|Specific Activity:||~ 100 U/mg (40oC, pH 5.0 on laminarin)|
|Unit Definition:||One Unit of endo-1,3-β-D-Glucanase activity is defined as the amount of enzyme required to release one µmole of glucose-reducing-sugar equivalents per minute in the presence of laminarin (10 mg/mL) in sodium acetate buffer (100mM), pH 5.0 at 40oC.|
|Application examples:||Applications in carbohydrate and biofuels research and in the food and feeds industries.|
High purity recombinant endo-1,3-β-Glucanase (barley) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Wu, D. T., Cheong, K. L., Deng, Y., Lin, P. C., Wei, F., Lv, X. J., Long, Z. R., Zhoa, J., Ma, S. C. & Li, S. P. (2015). Carbohydrate polymers, 134, 12-19.
Water-soluble polysaccharides from 51 batches of fruits of L. barbarum (wolfberry) in China were investigated and compared using saccharide mapping, partial acid hydrolysis, single and composite enzymatic digestion, followed by polysaccharide analysis by using carbohydrate gel electrophoresis (PACE) analysis and high performance thin layer chromatography (HPTLC) analysis, respectively. Results showed that multiple PACE and HPTLC fingerprints of partial acid and enzymatic hydrolysates of polysaccharides from L. barbarum in China were similar, respectively. In addition, results indicated that β-1,3-glucosidic, α-1,4-galactosiduronic and α-1,5-arabinosidic linkages existed in polysaccharides from L. barbarum collected in China, and the similarity of polysaccharides in L. barbarum collected from different regions of China was pretty high, which are helpful for the improvement of the performance of polysaccharides from L. barbarum in functional/health foods area. Furthermore, polysaccharides from Panax notoginseng, Angelica sinensis, and Astragalus membranaceus var. mongholicus were successfully distinguished from those of L. barbarum based on their PACE fingerprints. These results were beneficial to improve the quality control of polysaccharides from L. barabrum and their products, which suggested that saccharide mapping based on PACE and HPTLC analysis could be a routine approach for quality control of polysaccharides.Hide Abstract