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exo-1,3-β-D-Glucanase + β-Glucosidase

exo-1-3-beta-D-Glucanase beta-Glucosidase E-EXBGOS
Product code: E-EXBGOS

300 Units exo-1,3-β-glucanase / 60 Units β-glucosidase

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Content: 300 Units exo-1,3-β-glucanase / 60 Units β-glucosidase
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: β-Glucosidase, exo-1,3-β-Glucanase
EC Number: exo-1,3-β-glucanase:
CAZy Family: GH3, GH55
CAS Number: exo-1,3-β-glucanase: 9073-49-8
β-glucosidase: 9001-22-3
Synonyms: exo-1,3-β-Glucanase: glucan 1,3-beta-glucosidase; 3-beta-D-glucan glucohydrolase
β-Glucosidase: beta-glucosidase
Source: exo-1,3-β-Glucanase: Trichoderma virens,
β-Glucosidase: Aspergillus niger
Concentration: exo-1,3-β-Glucanase (100 U/mL) / β-Glucosidase (20 U/mL)
Expression: exo-1,3-β-D-Glucanase: From Trichoderma sp.
β-Glucosidase: From 
Aspergillus niger
Specificity: exo-1,3-β-glucanase: Successive hydrolysis of β-D-glucose units from the non-reducing ends of (1,3)-β-D-glucans, releasing α-glucose.
β-glucosidase: Hydrolysis of terminal, non-reducing β-D-glucosyl residues with release of β-D-glucose.
Unit Definition: exo-1,3-β-D-Glucanase: One Unit of exo-1,3-β-glucanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from laminarin (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40oC.
β-Glucosidase: One Unit of β-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl-β-D-glucpyranoside in sodium acetate buffer (100 mM), pH 4.0 at 40oC.
Temperature Optima: 40oC
pH Optima: 4
Application examples: For use in the determination of (1,3)(1,4) β-glucan.

High purity exo-1,3-β-D-Glucanase (Trichoderma sp.) + β-Glucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Certificate of Analysis
Safety Data Sheet
Generic tools to assess genuine carbohydrate specific effects on in vitro immune modulation exemplified by β-glucans.

Rieder, A., Grimmer, S., Aachmann, F. L., Westereng, B., Kolset, S. O. & Knutsen, S. H. (2013). Carbohydrate Polymers, 92(2), 2075-2083.

Even if carbohydrate preparations from plant/fungal sources have a high degree of purity, observed immune-stimulation may be caused by minute sample contaminations. Using the example of different β-glucans we present a range of analytical tools crucial for validation of possible immune-stimulatory effects. Two yeast (MacroGard and Zymosan) and one cereal β-glucan (CBG40) increased IL-8 secretion by HT-29 cells considerably. Degradation of the β-glucan samples with β-glucan specific enzymes did hardly influence the effect of Zymosan and CBG40 but significantly decreased the effect of MacroGard. Stimulation of IL-8 secretion by CBG40 and Zymosan was hence not due to their β-glucan content. Instead, the effect of the CBG40 sample was due to low levels of LPS despite the inability of the known LPS inhibitor Polymyxin B to supress its stimulatory effect. We conclude that targeted enzymatic degradation of samples is a powerful validation tool to investigate carbohydrate specific immune-modulation.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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