exo-1,3-β-D-Glucanase (Trichoderma virens)

Reference code: E-EXBGTV
SKU: 700004210

400 Units at 50oC;
~ 260 Units at 40oC

Content: 400 Units at 50oC;
~ 260 Units at 40oC
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 1 year under recommended storage conditions
Enzyme Activity: exo-1,3-β-Glucanase
EC Number:
CAZy Family: GH55
CAS Number: 9073-49-8
Synonyms: glucan 1,3-beta-glucosidase; 3-beta-D-glucan glucohydrolase
Source: Trichoderma virens
Molecular Weight: 81,700
Concentration: Supplied at ~ 200 U/mL
Expression: Recombinant from Trichoderma virens
Specificity: Successive hydrolysis of β-D-glucose units from the non-reducing ends of (1,3)-β-D-glucans, releasing α-glucose.
Specific Activity: ~ 90 U/mg (50oC, pH 4.5 on laminarin); 
~ 60 U/mg (40oC, pH 4.5 on laminarin)
Unit Definition: One Unit of exo-1,3-β-glucanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from laminarin (5 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
Temperature Optima: 50oC
pH Optima: 4.5
Application examples: Applications established in food and feeds, carbohydrate and biofuels industries.

High purity recombinant exo-1,3-β-D-Glucanase (Trichoderma virens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Comparative study on the structures of intra-and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins.

Zhang, S., Qiao, Z., Zhao, Z., Guo, J., Lu, K., Mayo, K. H. & Zhou, Y. (2021). International Journal of Biological Macromolecules, 181, 793-800.

Here, we compare the content and composition of polysaccharides derived from the mycelium (40.4 kDa intracellular polysaccharide, IPS) and culture (27.2 kDa extracellular polysaccharide, EPS) of Penicillium oxalicum. Their chemical structures investigated by IR, NMR, enzymolysis and methylation analysis indicate that both IPS and EPS are galactomannans composed of α-1,2- mannopyranose (Manp) and α-1,6-Manp in a backbone ratio of ~3:1, respectively, both decorated with β-l,5-galactofuranose (Galf) side chains. A few β-l,6-Galf residues were also detected in the IPS fraction. EPS and IPS have different molecular weights (Mw) and degrees of branching. IPS obtained by alkaline extraction of P. oxalicum have been reported to be galactofuranans, a composition different from our IPS. Up to now, there have been no reports on the fine structure of EPS. Our results of galectin-mediated hemagglutination demonstrate that IPS exhibits greater inhibitory effects on five galectins compared with EPS. In addition, we find that Galf, a five-membered ring form of galactose, can also inhibit galectins. IPS may provide a new source of galectin inhibitors. These results increase our understanding of structure-activity relationships of polysaccharides as galectin inhibitors.

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Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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