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|Formulation:||In 3.2 M ammonium sulphate|
|Stability:||> 4 years at 4oC|
|Concentration:||Supplied at ~ 3 U/mL|
|Expression:||Recombinant from Clostridium thermocellum|
|Specificity:||Random hydrolysis of N-acetyl-beta-D-glucosaminide (1-4)-beta-linkages in chitin and chitodextrins.|
|Specific Activity:||~ 0.3 U/mg (40oC, pH 6.2 on p-nitrophenyl-β-triacetylchitotriose)|
|Unit Definition:||One Unit of chitinase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol per minute from p-nitrophenyl-β-triacetylchitotriose (2.5 mM) in MES buffer (100 mM), pH 6.2 at 40oC.|
|Application examples:||For use in research.|
High purity chitinase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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