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Content:
6,000 Units Oligo-α-1,6-Glucosidase plus 6,000 Units β-galactosidase
Shipping Temperature:
Ambient
Storage Temperature:
4oC
Formulation:
In 3.2 M ammonium sulphate
Physical Form:
Suspension
Stability:
> 1 year under recommended storage conditions
Enzyme Activity:
Oligo-1,6-α-Glucosidase, β-Galactosidase
EC Number:
oligo-α-1,6-glucosidase: 3.2.1.10
β-galactosidase: 3.2.1.23
CAZy Family:
GH13, GH35
CAS Number:
oligo-α-1,6-glucosidase: 9032-15-9
β-galactosidase: 9013-11-2
Synonyms:
oligo-α-1,6-glucosidase: oligosaccharide 6-alpha-glucohydrolase
β-galactosidase: beta-D-galactoside galactohydrolase
Source:
oligo-α-1,6-glucosidase: Microbial
β-galactosidase: Aspergillus niger
Molecular Weight:
oligo-α-1,6-glucosidase: 67,300
β-galactosidase: 125,000
Concentration:
Oligo-α-1,6-glucosidase (2,000 U/mL) / β-galactosidase (2,000 U/mL)
Expression:
Oligo-α-1,6-glucosidase: Recombinant from a Microbial source
β-Glucosidase: Purified from Aspergillus niger
Specificity:
This enzyme mixture gives complete hydrolysis of isomaltose and lactose under the defined assay conditions, with no hydrolysis of the trisaccharide, β-D-Fruf-(2,1)-β-D-Fruf-(2,1)-β-D-Fruf.
Unit Definition:
Oligo-α-1,6-glucosidase: One Unit of oligo-α-1,6-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-glucopyranoside (5 mM) in sodium acetate buffer (50 mM), pH 4.5 at 40oC.
β-galactosidase: One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40oC.
Temperature Optima:
50oC
pH Optima:
4.5
Application examples:
Used for removal of isomaltose and lactose in the measurement of dietary fiber.

This product has been discontinued.

High purity Oligo-α-1,6-Glucosidase (microbial) plus β-Galactosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Safety Information

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