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Chapter 1: Theory of endo-1, 4-Beta-D-Xylanase Assay Procedure
Chapter 2: Buffers & Reagents
Chapter 3: Assay Procedure
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|Content:||200 Tablets or 1,000 Tablets|
|Stability:||> 10 years under recommended storage conditions|
|Substrate For (Enzyme):||endo-1,4-β-Galactanase|
|Reproducibility (%):||~ 5%|
High purity dyed and crosslinked Galactazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
For the assay of endo-1,4-β-D-galactanase. Containing AZCL-Galactan (potato).
Please note the video above shows the protocol for assay of endo-xylanase using xylazyme tablets. The procedure for the assay of endo-1,4-β-galactanase using Galactazyme Tablets is equivalent to this.
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de Vries, R. P., Pařenicová, L., Hinz, S. W. A., Kester, H. C. M., Beldman, G., Benen, J. A. E. & Visser, J. (2002). European Journal of Biochemistry, 269(20), 4985-4993.
The Aspergillus niger β-1,4-endogalactanase encoding gene (galA) was cloned and characterized. The expression of galA in A. niger was only detected in the presence of sugar beet pectin, D-galacturonic acid and L-arabinose, suggesting that galA is coregulated with both the pectinolytic genes as well as the arabinanolytic genes. The corresponding enzyme, endogalactanase A (GALA), contains both active site residues identified previously for the Pseudomonas fluorescens β-1,4-endogalactanase. The galA gene was overexpressed to facilitate purification of GALA. The enzyme has a molecular mass of 48.5 kDa and a pH optimum between 4 and 4.5. Incubations of arabinogalactans of potato, onion and soy with GALA resulted initially in the release of D-galactotriose and D-galactotetraose, whereas prolonged incubation resulted in D-galactose and D-galactobiose, predominantly. MALDI-TOF analysis revealed the release of L-arabinose substituted D-galactooligosaccharides from soy arabinogalactan. This is the first report of the ability of a β-1,4-endogalactanase to release substituted D-galacto-oligosaccharides. GALA was not active towards D-galacto-oligosaccharides that were substituted with D-glucose at the reducing end.Hide Abstract