Content: | 200 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | Minimum 1 year at 4oC. Check vial for details. |
Enzyme Activity: | Dehydrogenase |
EC Number: | 1.1.1.48 |
CAS Number: | 9028-54-0 |
Synonyms: | D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase |
Source: | Prokaryote |
Molecular Weight: | 36,659 |
Concentration: | Supplied at ~ 100 U/mL |
Expression: | Recombinant from a Soil prokaryote |
Specificity: |
Catalyses the reaction: D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+ Also acts on L-arabinose but more slowly. |
Specific Activity: | ~ 280 U/mg (25oC, pH 8.6 on D-galactose) |
Unit Definition: | One unit of galactose dehydrogenase activity is defined as the amount of enzyme required to convert one µmole of D-galactose to D-galactanate per minute in the presence of NAD+ in Tris-HCl buffer (93 mM), pH 8.6 at 25oC. |
Temperature Optima: | 40oC |
pH Optima: | 8.6 |
Application examples: | Applications for the measurement of D-galactose (and L-arabinose) in carbohydrate research and in the food, fermentation, wine, beverage and dairy industries. |
This product has been discontinued (read more).
High purity recombinant Galactose dehydrogenase (soil prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Assay for determination of free D-galactose and/or L-arabinose.
Charnock, S. C. & McCleary, B. V. (2006). Irish Patent No. S84280, UK Patent No. 1828407, German Patent No. 1828407, French Patent No. 1828407 and USA Patent No. US7, 785, 771/11/722,000.
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