Galactose dehydrogenase (soil prokaryote)

Reference code: E-GALDH

200 Units

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

200 Units

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

Minimum 1 year at 4^oC. Check vial for details.

Enzyme Activity:

Dehydrogenase

EC Number:

1.1.1.48

CAS Number:

9028-54-0

Synonyms:

D-galactose 1-dehydrogenase; D-galactose:NAD⁺ 1-oxidoreductase

Source:

Prokaryote

Molecular Weight:

36,659

Concentration:

Supplied at ~ 100 U/mL

Expression:

Recombinant from a Soil prokaryote

Specificity:

Catalyses the reaction:
D-galactose + NAD⁺ = D-galactono-1,4-lactone + NADH + H⁺Also acts on L-arabinose but more slowly.

Specific Activity:

~ 280 U/mg (25^oC, pH 8.6 on D-galactose)

Unit Definition:

One unit of galactose dehydrogenase activity is defined as the amount of enzyme required to convert one µmole of D-galactose to D-galactanate per minute in the presence of NAD⁺ in Tris-HCl buffer (93 mM), pH 8.6 at 25^oC.

Temperature Optima:

40^oC

pH Optima:

8.6

Application examples:

Applications for the measurement of D-galactose (and L-arabinose) in carbohydrate research and in the food, fermentation, wine, beverage and dairy industries.

This product has been discontinued (read more).

High purity recombinant Galactose dehydrogenase (soil prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

More analytical enzyme products available.

Publications

Cover image for publication: Determination of total dietary fibre and available carbohydrates

Assay for determination of free D-galactose and/or L-arabinose.

Charnock, S. C. & McCleary, B. V. (2006). Irish Patent No. S84280, UK Patent No. 1828407, German Patent No. 1828407, French Patent No. 1828407 and USA Patent No. US7, 785, 771/11/722,000.

Assay for determination of free D-galactose and/or L-arabinose.

Charnock, S. C. & McCleary, B. V. (2006). Irish Patent No. S84280, UK Patent No. 1828407, German Patent No. 1828407, French Patent No. 1828407 and USA Patent No. US7, 785, 771/11/722,000.