Galactose dehydrogenase - Galactose mutarotase

Reference code: E-GALMUT

1 mL; galactose dehydrogenase (200 U/mL) /
galactose mutarotase (4 mg/mL)

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

1 mL; galactose dehydrogenase (200 U/mL) / galactose mutarotase (4 mg/mL)

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

> 1 year under recommended storage conditions

Enzyme Activity:

Dehydrogenase

EC Number:

Galactose dehydrogenase: 1.1.1.48
Galactose mutarotase: 5.1.3.3

CAS Number:

Galactose dehydrogenase: 9028-54-0
Galactose mutarotase: 9031-76-9

Synonyms:

Galactose dehydrogenase: D-galactose 1-dehydrogenase; D-galactose:NAD⁺ 1-oxidoreductase
Galactose mutarotase: aldose 1-epimerase; aldose 1-epimerase

Source:

Escherichia coli

Expression:

Recombinant from Escherichia coli

Specificity:

Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by β-galactose mutarotase (GMR) (1).

(1) α-D-Galactose ↔ β-D-Galactose.

The β-D-Galactose is oxidised by NAD⁺ to D-galactonic acid in the presence of β-galactose dehydrogenase (GALDH) at pH 7.5 (2).

(2) β-D-Galactose + NAD⁺ → D-galactonic acid + NADH + H⁺

Specific Activity:

Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25^oC
Galactose mutarotase: ~ 4 mg/mL

Unit Definition:

One Unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD⁺ per minute at pH 8.6, at 25^oC.

Temperature Optima:

25^oC

pH Optima:

8.6

Application examples:

Used for the rapid measurement of D-galactose or L-arabinose in the L-Arabinose/D-Galactose Assay Kit (K-ARGA). Also used in the measurement of lactose via D-galactose in the Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR).

This product has been discontinued.

High purity Galactose dehydrogenase (Escherichia coli) / Galactose mutarotase (Escherichia coli) for use in research, biochemical enzyme assays and analytical testing applications.

Can be employed for the rapid determination of D-galactose or L-arabinose. See Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR) for full details.

For use under Patent No. US 7,785,771 B2 and EP1 828 407 (GB, FR, IE, DE)

Browse a wide range of enzymes for analytical applications.

Publications

Cover image for publication: Determination of total dietary fibre and available carbohydrates

Assay for determination of free D-galactose and/or L-arabinose.

Charnock, S. C. & McCleary, B. V. (2006). Irish Patent No. S84280, UK Patent No. 1828407, German Patent No. 1828407, French Patent No. 1828407 and USA Patent No. US7, 785, 771/11/722,000.

Assay for determination of free D-galactose and/or L-arabinose.

Charnock, S. C. & McCleary, B. V. (2006). Irish Patent No. S84280, UK Patent No. 1828407, German Patent No. 1828407, French Patent No. 1828407 and USA Patent No. US7, 785, 771/11/722,000.

Method for the Direct Determination of Available Carbohydrates in Low-Carbohydrate Products Using High-Performance Anion Exchange Chromatography.

Ellingson, D., Potts, B., Anderson, P., Burkhardt, G., Ellefson, W., Sullivan, D., Jacobs, W. & Ragan, R. (2010). Journal of AOAC International, 93(6), 1897-1904.

Method for the Direct Determination of Available Carbohydrates in Low-Carbohydrate Products Using High-Performance Anion Exchange Chromatography.

Ellingson, D., Potts, B., Anderson, P., Burkhardt, G., Ellefson, W., Sullivan, D., Jacobs, W. & Ragan, R. (2010). Journal of AOAC International, 93(6), 1897-1904.

An improved method for direct determination of available carbohydrates in low-level products has been developed and validated for a low-carbohydrate soy infant formula. The method involves modification of an existing direct determination method to improve specificity, accuracy, detection levels, and run times through a more extensive enzymatic digestion to capture all available (or potentially available) carbohydrates. The digestion hydrolyzes all common sugars, starch, and starch derivatives down to their monosaccharide components, glucose, fructose, and galactose, which are then quantitated by high-performance anion-exchange chromatography with photodiode array detection. Method validation consisted of specificity testing and 10 days of analyzing various spike levels of mixed sugars, maltodextrin, and corn starch. The overall RSD was 4.0 across all sample types, which contained within-day and day-to-day components of 3.6 and 3.4, respectively. Overall average recovery was 99.4 (n = 10). Average recovery for individual spiked samples ranged from 94.1 to 106 (n = 10). It is expected that the method could be applied to a variety of low-carbohydrate foods and beverages.

Link to Article