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Phosphate Assay Kit

Product code: K-PHOS

100 assays (manual) / 400 assays (auto-analyser)

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Content: 100 assays (manual) / 400 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Phosphate
Assay Format: Spectrophotometer, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 360
Signal Response: Increase
Linear Range: 0.1 to 10 μg of phosphate per assay
Limit of Detection: 0.16 mg/L
Reaction Time (min): ~ 20 min
Application examples: Processed foods and drinks, bakery products, dairy products, waste water samples, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, etc.).

The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.

This method is suitable for analysis using spectrophotometer and auto-analyser.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.

Need other products? See our complete list of assay kits.

Scheme-K-PHOS PHOS Megazyme

  • Chemically defined detection method
  • All reagents stable for > 2 years after preparation 
  • Rapid reaction (~ 20 min)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included
  • Suitable for manual and auto-analyser formats
Certificate of Analysis
Safety Data Sheet
Assay Protocol Data Calculator
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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