Phosphate Assay Kit

Reference code: K-PHOS

100 assays (manual) / 400 assays (auto-analyser)

This product has been discontinued

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Product Instruction

SDS

Mega-Calc

Certificate of Analysis

Content:

100 assays (manual) / 400 assays (auto-analyser)

Shipping Temperature:

Ambient

Storage Temperature:

Short term stability: 2-8^oC,
Long term stability: See individual component labels

Stability:

> 2 years under recommended storage conditions

Analyte:

Phosphate

Assay Format:

Spectrophotometer, Auto-analyser

Detection Method:

Absorbance

Wavelength (nm):

360

Signal Response:

Increase

Linear Range:

0.1 to 10 μg of phosphate per assay

Limit of Detection:

0.16 mg/L

Reaction Time (min):

~ 20 min

Application examples:

Processed foods and drinks, bakery products, dairy products, waste water samples, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, etc.).

Advantages

Chemically defined detection method
All reagents stable for > 2 years after preparation
Rapid reaction (~ 20 min)
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual and auto-analyser formats

This product has been discontinued.

The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.

This method is suitable for analysis using spectrophotometer and auto-analyser.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.

Need other products? See our complete list of assay kits.

Scheme-K-PHOS PHOS Megazyme