Hydrogen Peroxide Assay Kit (Megaplex Red)

Reference code: K-MRH2O2

1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

This product has been discontinued

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Product Instruction

SDS

Mega-Calc

Certificate of Analysis

Content:

1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

Shipping Temperature:

Ambient

Storage Temperature:

Short term stability: 2-8^oC,
Long term stability: See individual component labels

Stability:

> 2 years under recommended storage conditions

Analyte:

Hydrogen Peroxide

Assay Format:

Spectrophotometer, Microplate, Auto-analyser

Detection Method:

Absorbance, Fluorescence

Wavelength (nm):

(Fluorescence) 530-560 (ex), ~ 590 (em)
(Absorbance) 570

Signal Response:

Increase

Linear Range:

(Fluorescence) 0.0034-0.17 µg of H₂O₂per assay (0.1-5 µM in-assay)
(Absorbance) 0.034-0.68 µg of H₂O₂per assay (1-20 µM in-assay)

Limit of Detection:

(Fluorescence) 0.0034 ug/mL
(Absorbance) 0.25 ug/mL

Reproducibility (%):

(Fluorescence) ~ 8%
(Absorbance) ~ 8%

Reaction Time (min):

(Fluorescence) 30 min
(Absorbance) 30 min

Application examples:

Food and beverage samples such as milk or milk based products, wine, fruit juices, paper (and cardboard), waste treatment samples and other materials (e.g. biological cultures etc.).

Advantages

Simple format
Very competitive price (cost per test)
Ability to run in fluorescence or absorbance mode
User friendly - Detailed protocol provided for the creation of calibration curve and the calculation of concentration in fluorescence mode. Single point standard for quicker and simpler analysis in absorbance mode.
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Suitable for manual, microplate and auto-analyser formats
All reagents stable for > 2 years

This product has been discontinued.

The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H₂O₂ in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.

Display all assay test kits available.

Fluorometric/UV method for the determination of H₂O₂ content in a variety of samples.

Scheme-K-MRH2O2 MRH2O2 Megazyme

Publications

Authenticity of Honey: Characterization, Bioactivities and Sensorial Properties.

Escuredo, O. & Seijo, M. C. (2022). Foods, 11(9), 1301.

Authenticity of Honey: Characterization, Bioactivities and Sensorial Properties.

Escuredo, O. & Seijo, M. C. (2022). Foods, 11(9), 1301.

Honey is a natural product well known for its beneficial properties, which depend on its composition. This composition of honey is related to its botanical and geographical origin, as well as its environmental and management conditions. Honey contains a wide spectrum of components, many of which are phytochemicals found in plants; these include compounds with highly demonstrated antimicrobial, antioxidant and anti-inflammatory properties. Studies about the composition, properties, and botanical and geographical origins of different honey types are relevant in society; they are especially relevant in the honey market, as they provide knowledge to guarantee honey authenticity.

Link to Article