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Protein Digestibility Assay Kit

Product code: K-PDCAAS
€296.00

50 assays per kit

Prices exclude VAT

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Content: 50 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Protein Digestibility
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 570
Signal Response: Increase
Total Assay Time: ~ 6 h (over 2 days)
Application examples: Protein containing foods and feeds.
Method recognition: Novel Method (Under Patent: U.S. Pat. No. 9,738,920)

The Protein Digestibility Assay Kit (K-PDCAAS) is used for in vitro measurement of the Animal-Safe Accurate Protein Quality Score (ASAP-Quality Score Method) developed under patent by Medallion Labs.  This digestibility score, in conjunction with the essential amino acid profile plus protein and moisture content of the sample, is used to calculate the Protein Digestibility Corrected Amino Acid Score (PDCAAS) value.

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Click here to view the advantages of this PDCAAS method

Scheme-K-PDCAAS PDCAAS Megazyme

Advantages
  • U.S. Pat. No. 9,738,920 
  • Control samples included
    ASAP-Quality Score offers:
  • Animal free 
  • High correlation to rat digestibility 
  • Much less expensive than rat model 
  • Faster turnaround time for results
Documents
Certificate of Analysis
Safety Data Sheet
FAQs Assay Protocol Data Calculator
Publications
Publication

Characterization, functional and biological value of protein-enriched defatted meals from sacha inchi (Plukenetia volubilis) and chocho (Lupinus mutabilis).

Cordero-Clavijo, L. M., Serna-Saldívar, S. O., Lazo-Vélez, M. A., González, J. F. A., Panata-Saquicilí, D. & Briones-Garcia, M. (2021). Journal of Food Measurement and Characterization, 15, 5071–5077.

A deficit in protein intake can severely halt children growth, and is associated with both structural and functional pathologies of the brain, as well as increase the risk of contracting infectious diseases. Protein-enriched defatted meals from sacha inchi (Plukenetia volubilis) (sdSI) and chocho (Lupinus mutabilis) (sdCH) were obtained by a solvent defatting procedure. The concentration of protein was higher in sdSI (64%) than in sdCH (60%). Lysine and tryptophan were the limiting amino acid (AA) in each case. However, the AA profile of the protein meals met daily intake FAO requirements for adults, while their blends met the requirements for infants. The sdSI had an in vitro digestibility of 100. Regarding functional properties, sdSI presented higher values for water absorption index (3.5), emulsifying activity (56%), and foaming capacity (167%), while the sdCH had a slightly better oil absorption capacity (2.8). However, when mixing sdSI with sdCH some improvements in functional properties were observed. The nutritional and functional properties of the mixtures of meals can be used to develop protein-enriched foods and beverages.

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Publication

In Vitro Digestibility and Antioxidant Activity of Plant Protein Isolate and Milk Protein Concentrate Blends.

Khalesi, M. & FitzGerald, R. J. (2021). Catalysts, 11(7), 787.

The replacement of animal with plant proteins in human diets has been increasing in recent years. The impact of blending milk protein concentrate (MPC) with protein isolates from soy (SPI), rice (RPI) and pea (PPI) on the in vitro digestibility and antioxidant activity of the resultant blends was investigated. Different plant protein–MPC blends (i.e., SPI–MPC (25:75), RPI–MPC (50:50) and PPI–MPC (25:75)) were analyzed. The lowest protein digestibility corrected amino acid score (PDCAAS) was associated with RPI (0.70), while the blends had PDCAAS values above 1.00 demonstrating the high digestibility of the proteins in the blends studied. An in vitro simulated gastrointestinal digestion was carried out on the samples. The degree of hydrolysis and gel permeation high performance liquid chromatography profiles showed that the SPI–MPC blend was more extensively digested in the gastric phase compared with the two other blends, while the PPI–MPC and RPI–MPC blends were mainly digested during the intestinal phase. The SPI–MPC digested blend had the highest 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity having a half maximal effective concentration (EC50) of 0.10 ± 0.01 mg/mL. The findings show that blends of plant protein with MPC had higher in vitro digestibility and antioxidant activity compared to the individual plant protein isolates.

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Safety Information
Symbol : GHS07, GHS08
Signal Word : Danger
Hazard Statements : H315, H319, H334, H335
Precautionary Statements : P261, P264, P271, P280, P284, P302+P352
Safety Data Sheet
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