The product has been successfully added to your shopping list.

Glucuronoyl Esterase Assay Kit

Product code: K-GEUX3
€403.00

100 assays per kit

Prices exclude VAT

This product is currently unavailable, please contact cs@megazyme.com.

Content: 100 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Glucuronoyl Esterase
Assay Format: Spectrophotometer, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400
Signal Response: Increase
Reproducibility (%): ~ 3%
Total Assay Time: 10 min
Application examples: Fermentation broths, industrial enzyme preparations and biofuels research.
Method recognition: Novel method

The Glucuronoyl Esterase Assay Kit provides a simple robust method for the measurement of glucuronoyl esterase. The assay kit employs a novel colourimetric reagent, termed GEUX3 that is highly specific for glucuronoyl esterase.

Browse more enzyme activity assay kits

View K-GEUX3 Product Flyer

Scheme-K-GEUX3 GEUX3 Megazyme

Advantages
  • Cost effective
  • All reagents stable for > 2 years 
  • Specific for glucuronoyl esterase
  • Suitable for use in crude enzyme preparations
  • Suitable for manual and auto-analyser formats
  • Standard included
Documents
Certificate of Analysis
Safety Data Sheet
Assay Protocol Data Calculator
Publications
Publication

Structural and functional investigation of a fungal member of carbohydrate esterase family 15 with potential specificity for rare xylans.

Mazurkewich, S., Scholzen, K. C., Brusch, R. H., Poulsen, J. C., Theibich, Y., Hüttner, S., Olsson, L., Larsbrink, J. & Lo Leggio, L. (2023). Acta Crystallographica Section D: Structural Biology, 79(6), 545-555.

In plant cell walls, covalent bonds between polysaccharides and lignin increase recalcitrance to degradation. Ester bonds are known to exist between glucuronic acid moieties on glucuronoxylan and lignin, and these can be cleaved by glucuronoyl esterases (GEs) from carbohydrate esterase family 15 (CE15). GEs are found in both bacteria and fungi, and some microorganisms also encode multiple GEs, although the reason for this is still not fully clear. The fungus Lentithecium fluviatile encodes three CE15 enzymes, of which two have previously been heterologously produced, although neither was active on the tested model substrate. Here, one of these, LfCE15C, has been investigated in detail using a range of model and natural substrates and its structure has been solved using X-ray crystallography. No activity could be verified on any tested substrate, but biophysical assays indicate an ability to bind to complex carbohydrate ligands. The structure further suggests that this enzyme, which possesses an intact catalytic triad, might be able to bind and act on more extensively decorated xylan chains than has been reported for other CE15 members. It is speculated that rare glucuronoxylans decorated at the glucuronic acid moiety may be the true targets of LfCE15C and other CE15 family members with similar sequence characteristics.

Hide Abstract
Safety Information
Symbol : GHS06, GHS08, GHS09
Signal Word : Danger
Hazard Statements : H300, H334, H411
Precautionary Statements : P261, P264, P270, P273, P284, P301+P310, P304+P340, P342+P311, P501
Safety Data Sheet
Customers also viewed
Glycerol Assay Kit K-GCROL GCROL
Glycerol Assay Kit
€134.00
Phytase Assay Kit K-PHYTASE PHYTASE
Phytase Assay Kit
€375.00
Formaldehyde Assay Kit K-FRHYD FRHYD
Formaldehyde Assay Kit
€177.00
Chitosanpentaose O-CHIS5
Chitosanpentaose
€0.01
Chitosantriose O-CHIS3
Chitosantriose
€0.01
Chitosanbiose O-CHIS2
Chitosanbiose
€0.01
Digestible and Resistant Starch Assay Kit K-DSTRS DSTRS
Digestible and Resistant Starch Assay Kit
€272.00
L-Malic Acid Assay Kit Manual Format K-LMAL LMAL
L-Malic Acid Assay Kit (Manual Format)
€210.00