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D-Glucose Assay Kit (Megaplex Red)

Product code: K-MRGLUC
€300.00

100 assays (cuvette) / 1000 assays (microplate) / 400 assays (auto-analyser)

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Content: 100 assays (cuvette) / 1000 assays (microplate) / 400 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: D-Glucose
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance, Fluorescence
Wavelength (nm): (Absorbance) 570
(Fluorescence) 530-560 (ex), ~ 590 (em)
Signal Response: Increase
Linear Range: (Absorbance) 5-50 μM (0.9-9 mg/L) of D-glucose in-assay
(Fluorescence) 0.5-10 μM (0.09-1.8 mg/L) of D-glucose in-assay
Limit of Detection: 0.68 μM (0.12 mg/L)
Reproducibility (%): ~ 7%
Reaction Time (min): ~ 30 min
Application examples: Food and beverage samples such as wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery products, candies, fruit and vegetables. Other samples such as tobacco, cosmetics, pharmaceuticals (e.g. infusions), feed, paper (and cardboard) and other materials (e.g. biological cultures, samples, etc.).

The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured colourimetrically at 570 nm or in fluorescence mode* using excitation between 530-560 nm and emission at ~ 590 nm.

*The excitation and emission maxima of Megaplex Red are 570 nm and 585 nm respectively.

See more related mono/disaccharide assay kit products. 

UV/Fluorometric method for the determination of D-glucose content in a variety of samples.

 Scheme-K-MRGLUC MRGLUC Megazyme

Advantages
  • Very competitive price (cost per test)
  • Simple format
  • Ability to run in absorbance or fluorescence mode
  • All reagents stable for > 2 years
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • User friendly - No standard curve required for a quicker and simpler analysis.
  • Suitable for manual, microplate and auto-analyser formats
Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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