Glucose-6-phosphate dehydrogenase
(Escherichia coli)
Reference code: E-GPDHEC
5,000 Units
This product has been discontinued
Content:
5,000 Units
Shipping Temperature:
Ambient
Storage Temperature:
2-8oC
Formulation:
In 3.2 M ammonium sulphate
Physical Form:
Suspension
Stability:
Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity:
Dehydrogenase
EC Number:
1.1.1.49
CAS Number:
9001-40-5
Synonyms:
glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase
Source:
Escherichia coli
Molecular Weight:
56,770
Concentration:
Supplied at ~ 1,250 U/mL
Expression:
Recombinant from Escherichia coli
Specificity:
Catalyses the reaction:
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
Specific Activity:
~ 160 U/mg (25oC, pH 7.6 on D-glucose-6-phosphate)
Unit Definition:
One Unit of Glucose-6-phosphate dehydrogenase activity is defined as the amount of enzyme required to convert one µmole of glucose 6-phosphate (5.0 mM) to 6-phosphogluconate per minute in the presence of NADP+ in imidazole buffer (168 mM), pH 7.6 at 25oC.
Temperature Optima:
25oC
pH Optima:
7.6
Application examples:
Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.
This product has been discontinued (read more).
High purity recombinant Glucose-6-phosphate dehydrogenase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Safety Information
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