Reference code:
E-GPDHEC
5,000 Units
This product has been discontinued
Content: | 5,000 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | Minimum 1 year at 4oC. Check vial for details. |
Enzyme Activity: | Dehydrogenase |
EC Number: | 1.1.1.49 |
CAS Number: | 9001-40-5 |
Synonyms: | glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase |
Source: | Escherichia coli |
Molecular Weight: | 56,770 |
Concentration: | Supplied at ~ 1,250 U/mL |
Expression: | Recombinant from Escherichia coli |
Specificity: |
Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ |
Specific Activity: | ~ 160 U/mg (25oC, pH 7.6 on D-glucose-6-phosphate) |
Unit Definition: | One Unit of Glucose-6-phosphate dehydrogenase activity is defined as the amount of enzyme required to convert one µmole of glucose 6-phosphate (5.0 mM) to 6-phosphogluconate per minute in the presence of NADP+ in imidazole buffer (168 mM), pH 7.6 at 25oC. |
Temperature Optima: | 25oC |
pH Optima: | 7.6 |
Application examples: | Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
This product has been discontinued (read more).
High purity recombinant Glucose-6-phosphate dehydrogenase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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