Reference code:
E-GPDH5
5,000 Units
This product has been discontinued
Content: | 5,000 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | > 4 years at 4oC |
Enzyme Activity: | Dehydrogenase |
EC Number: | 1.1.1.49 |
CAS Number: | 9001-40-5 |
Synonyms: | glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase |
Source: | Leuconostoc mesenteroides |
Molecular Weight: | 54,441 |
Concentration: | Supplied at ~ 2,000 U/mL |
Expression: | Purified from Leuconostoc mesenteroides |
Specificity: |
Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ |
Specific Activity: | ~ 800 U/mg (30oC, pH 7.8 on glucose-6-phosphate) |
Unit Definition: | One Unit of Glucose-6-phosphate dehydrogenase activity is defined as the amount of enzyme required to convert one µmole of glucose 6-phosphate (3.3 mM) to 6-phosphogluconate per minute in the presence of NAD+ in Tris-HCl buffer (51 mM), pH 7.8 at 30oC. |
Temperature Optima: | 40oC |
pH Optima: | 7.8 |
Application examples: | Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
This product has been discontinued (read more).
High purity Glucose-6-phosphate dehydrogenase (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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