Glucoamylase P (H. resinae)
340 Units at 40oC;
~ 800 Units at 60oC
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| Content: |
340 Units at 40oC; ~ 800 Units at 60oC |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Amyloglucosidase |
| EC Number: | 3.2.1.3 |
| CAZy Family: | GH15 |
| CAS Number: | 9032-08-0 |
| Synonyms: | amyloglucosidase; exo-1,4-alpha-glucosidase; glucan 1,4-alpha-glucosidase; glucoamylase |
| Source: | Hormoconis resinae |
| Molecular Weight: | 65,400 |
| Concentration: | Supplied at ~ 170 U/mL |
| Expression: | Recombinant from Hormoconis resinae |
| Specificity: | Hydrolysis of terminal non-reducing α-1,4-D-glycosidic bonds in α-1,4-D-glucans with “debranching activity” (hydrolysis of α-1,6-D-glycosidic bonds) in substrates such as starch and pullulan. |
| Specific Activity: |
~ 60 U/mg (40oC, pH 4.5 on soluble starch); ~ 160 U/mg (60oC, pH 4.5 on soluble starch) |
| Unit Definition: | One Unit of glucoamylase activity is defined as the amount of enzyme required to release one µmole of β-D-glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.5. |
| Temperature Optima: | 60oC |
| pH Optima: | 4.5 |
| Application examples: | Applications in carbohydrate and biofuels research and in the food and feeds industries. |
High purity recombinant Glucoamylase P (Hormoconis resinae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Park, J., Rho, S. J. & Kim, Y. R. Cereal Chemistry, In Press.
Background and objectives: Cycloamylose (CA), a promising encapsulating agent, was efficiently produced from high amylose corn starch (HACS, HYLON ® VII) by sequentially‐combined enzyme treatment of isoamylase and Thermus aquaticus 4‐α-glucanotransferase (TAαGT). The CA production performance of HACS was compared with that of rice starch, especially from the viewpoint of differences in their molecular characteristics. Findings: The maximum conversion yield of CA from HACS reached 76.35%, which was 2.4‐times higher than that from rice starch (31.36%). The degree of polymerization (DP) of CA produced from HACS ranged from 7 to 41, where a major portion lied in DP 23-41, with DP 26 showing the highest yield. In contrast, CA produced from rice starch showed a relatively larger amount of smaller cyclic glucans (DP 6-16). Conclusion: The significantly high production yield and DP profile of CA were attributed to high apparent amylose content and long average amylopectin branch chain length of HACS compared to those rice starch. Significance and novelty: This study can provide a better understanding of CA production depending on starch molecular characteristics, and attract industrial consideration in utilizing HACS for CA production.
Hide Abstract| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
