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Amyloglucosidase (Aspergillus niger)
Glycerol Free

Amyloglucosidase (Aspergillus niger) Glycerol Free
Product code: E-AMGDFNG-20ML

Content:

€147.00

20 mL

Prices exclude VAT

Available for shipping

Content: 20 mL or 50 mL
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In buffered solution
Physical Form: Solution
Stability: > 4 years at 4oC
Enzyme Activity: Amyloglucosidase
EC Number: 3.2.1.3
CAZy Family: GH15
CAS Number: 9032-08-0
Synonyms: glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase
Source: Aspergillus niger
Molecular Weight: 143,500
Expression: From Aspergillus niger
Specificity: Hydrolysis of terminal (1,4)-linked α-D-glucose residues successively from non-reducing ends of the chains with release of β-D-glucose.
Specific Activity: ~ 36 U/mg (40oC, pH 4.5 on soluble starch);
~ 400 U/mL on p-nitrophenyl β-maltoside;
~ 6,520 U/mL on soluble starch
Unit Definition: One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) at pH 4.5 at 40oC.
Temperature Optima: 70oC
pH Optima: 4
Application examples: This enzyme is recommended for use in Total Dietary Fiber analytical procedures and the Megazyme Total Starch test method. The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method).

High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

Data booklets for each pack size are located in the Documents tab.

View Megazyme’s latest Guide for Dietary Fiber Analysis.

Safety Information
Symbol : GHS08
Signal Word : Danger
Hazard Statements : H334
Precautionary Statements : P261, P284, P304+P340, P342+P311, P501
Safety Data Sheet
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