4 g - 36,000 Units/g
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|Content:||4 g - 36,000 Units/g|
|Storage Temperature:||Below -10oC|
|Formulation:||Supplied as a lyophilised powder|
|Stability:||Minimum 1 year at < -10oC. Check vial for details.|
|Synonyms:||4-alpha-D-glucan glucohydrolase; glucoamylase|
|Expression:||From Aspergillus niger|
|Specificity:||Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins.|
approx. 36 U/mg (40oC, pH 4.5, soluble starch as substrate).
3,100 U/g on p-nitrophenyl β-maltoside;
36,000 U/g on soluble starch
|Unit Definition:||One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40oC.|
|Method recognition:||AOAC Method 2000.11, AOAC Method 2016.06 and GB Standards 5009.255-2016|
High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Amyloglucosidase. From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fiber methods. Specifically prepared for use in AOAC Method 2001.03 which requires enzymes free of glycerol. This AMG is used in combination with thermostable α-amylase (Cat. No. E-BLAAM) and protease powder (Cat. No. E-BSPRPD). For use, the AMG is dissolved in water at a rate of 1 g/10 mL and 0.2 mL is used per assay. Electrophoretically homogeneous. Lyophilised powder.
Validation of Methods