|Content:||500 assays per kit|
Short term stability: Ambient,
Long term stability: See individual component labels
|Stability:||> 2 years under recommended storage conditions|
|Reaction Time (min):||~ 30 min|
|Application examples:||Food ingredients (e.g. citrus fruit and apple) and other materials.|
The Pectin Identification Assay Kit is suitable for the identification of pectin in food ingredients. This kit now employs a new pectate lyase from Aspergillus niger.
See the complete list of our polysaccharide assay kit products.
- Very cost effective
- All reagents stable for > 2 years after preparation
- Only enzymatic kit available
- Simple format
- Standard included
(Aspergillus aculeatus) E-PGALPC - endo-Polygalacturonanase
(Pectobacterium carotovorum) E-PCLYAN - Pectate Lyase (Aspergillus sp.) E-PCLYAN2 - Pectate Lyase (Aspergillus sp.) E-PLYCJ - Pectate lyase (Cellvibrio japonicus) E-EXPGA - exo-Polygalacturonase (Yersinia enterocolitica)
Udeh, C. B., Ameh, J. B., Ado, S. A. & Okoduwa, S. I. R. (2017). Journal of Biotechnology Research, 3(3), 15-24.
Background: In the present study, optimized cultural conditions for enhanced production of xylanase from local soil isolate of Trichoderma species, using water hyacinth as a substrate in submerged culture fermentation is presented. Method: The Megazyme assay method was used for endo 1, 4-β-xylanase using Azo-xylan (Birchwood). Results: A continuous increase in xylanase production was observed with increasing level of substrate concentration in the medium and highest production was obtained with water hyacinth at 6% w/v level. Maximum xylanase production was achieved with a pH 5.0, incubation temperature of 30°C and agitation rate of 150 rpm. The highest production was achieved on day five of fermentation at optimum parameters under study. Conclusion: The study showed that production of xylanase can be cost effective using water hyacinth and can be implored on large scale for industrial applications.Hide Abstract