endo-Polygalacturonanase
(Pectobacterium carotovorum)

Reference code: E-PGALPC
SKU: 700004232

5,000 Units

Content: 5,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 1 year under recommended storage conditions
Enzyme Activity: endo-Polygalacturonase
EC Number: 3.2.1.15
CAZy Family: GH28
CAS Number: 9032-75-1
Synonyms: polygalacturonase; (1→4)-alpha-D-galacturonan glycanohydrolase
Source: Pectobacterium carotovorum
Molecular Weight: 39,818
Concentration: Supplied at ~ 14,000 U/mL
Expression: Recombinant from Pectobacterium carotovorum
Specificity: Random hydrolysis of α-1,4-D-galactosiduronic linkages in pectate and polygalacturonans.
Specific Activity: ~ 400 U/mg (40oC, pH 5.5 on polygalacturonic acid)
Unit Definition: One Unit of endo-polygalacturonanase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (5 mg/mL) per min in sodium acetate buffer (100 mM), pH 5.5 at 40oC.
Temperature Optima: 50oC
pH Optima: 6
Application examples: Applications in carbohydrate research and in the food industry.

High purity endo-Polygalacturonanase (Pectobacterium carotovorum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Documents
Certificate of Analysis
Safety Data Sheet
Data Sheet
Publications
Publication

Gelation behavior between Nicandra physalodes (Linn.) Gaertn. pectin and Ca2+ is governed by degree and pattern of methylesterification.

Zhang, H., Song, Z., Gong, T., Meng, Y., Wang, X., Zhang, S. & Guo, Y. (2024). Food Hydrocolloids, 149, 109640.

Nicandra physalodes (Linn.) Gaertn. Pectin (NPGP) is a new type of natural low methoxy pectin (LMP), however, the relatively poor gelling capacity of NPGP and neutral calcium limits its applicability in the food industry. In this study, we improved the gelling capacity of NPGP, and the impact of the degree and pattern of methyl esterification on NPGP gelation behavior triggered by neutral calcium was investigated. To achieve this goal, NPGP was treated with pectin methyl esterase (PME, 30°C, pH 4.5) for various time. This modification decreased the degree of methyl esterification (DM) and generated different patterns of methyl ester distribution, quantified as the degree of blockiness (DB). The obtained PME-NPGPs possess the ability to form self-supporting gels in the presence of CaCl2, with the highest gel hardness being 331.98 g. By estimating the DM and quantifying the DB of PME-NPGP, the DB value of PME-NPGP was increased from 17.74% to 31.84% with DM decreasing from 33.09% to 17.37%, indicating a shift toward a more blockwise distribution of nonmethylated GalA in NPGP. Additionally, adsorption isotherms for Ca2+ on de-esterified NPGP were determined, enabling the quantification of its cation binding capacity and interaction energy. Reducing DM and increasing DB of NPGP were found to enhance NPGP's binding capacity to Ca2+. This research provides an important foundation for NPGP food processing, and the obtained PME-NPGP can be promising in the fields of food gel and drug delivery.

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Publication

The model polysaccharide potato galactan is actually a mixture of different polysaccharides.

Feng, Z., Lin, Z., Tang, H., Geng, J., Hu, Y., Mayo, K. H., Tai, G. & Zhou, Y. (2023). Carbohydrate Polymers, 313, 120889.

Commercially-supplied potato galactan (PG) is widely used as a model polysaccharide in various bioactivity studies. However, results using this galactan are not always consistent with the stated composition. Here, we assessed its composition by fractionating this commercial PG and purified its primary components: PG-A, PG-B and PG-Cp with weight-averaged molecular weights of 430, 93, and 11.3 kDa, respectively. PG-Cp consists of free β-1,4-galactan chains, whereas PG-A and PG-B are type I rhamnogalacturonans with long β-1,4-galactan side chains of up to 80 Gal residues and short β-1,4-galactan side chains of 0 to 3 Gal residues that display a “trees in lawn” pattern. Structures of these polysaccharides correlate well with their activities in terms of galectin-3 binding and gut bacterial growth assays. Our study clarifies the confusion related to commercial PG, with purified fractions serving as better model polysaccharides in bioactivity investigations.

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Publication

MUR1‐mediated cell‐wall fucosylation is required for freezing tolerance in Arabidopsis thaliana.

Panter, P. E., Kent, O., Dale, M., Smith, S. J., Skipsey, M., Thorlby, G., Cummins, I., Ramsey, N., Begum, R. A., Sanhueza., Fry, S. C., Knight, M. R. & Fry, S. C. (2019). New Phytologist, 224(4), 1518-1531.

Summary: Forward genetic screens play a key role in the identification of genes contributing to plant stress tolerance. Using a screen for freezing sensitivity, we have identified a novel freezing tolerance gene, SENSITIVE‐TO‐FREEZING8, in Arabidopsis thaliana. We identified SFR8 using recombination‐based mapping and whole‐genome sequencing. As SFR8 was predicted to have an effect on cell wall composition, we used GC‐MS and polyacrylamide gel electrophoresis to measure cell‐wall fucose and boron (B)‐dependent dimerization of the cell‐wall pectic domain rhamnogalacturonan II (RGII) in planta. After treatments to promote borate‐bridging of RGII, we assessed freeze‐induced damage in wild‐type and sfr8 plants by measuring electrolyte leakage from freeze‐thawed leaf discs. We mapped the sfr8 mutation to MUR1, a gene encoding the fucose biosynthetic enzyme GDP‐d‐mannose‐4,6‐dehydratase. sfr8 cell walls exhibited low cell‐wall fucose levels and reduced RGII bridging. Freezing sensitivity of sfr8 mutants was ameliorated by B supplementation, which can restore RGII dimerization. B transport mutants with reduced RGII dimerization were also freezing‐sensitive. Our research identifies a role for the structure and composition of the plant primary cell wall in determining basal plant freezing tolerance and highlights the specific importance of fucosylation, most likely through its effect on the ability of RGII pectin to dimerize.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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