Glucuronoyl Esterase (Ruminococcus flavefaciens)
Reference code: E-GERF
250 Units
This product has been discontinued
Content:
250 Units
Shipping Temperature:
Ambient
Storage Temperature:
2-8oC
Formulation:
In 3.2 M ammonium sulphate
Physical Form:
Suspension
Stability:
> 4 years at 4oC
Enzyme Activity:
Esterase
EC Number:
3.1.1.B11
CAZy Family:
CE15
CAS Number:
9016-18-6
Synonyms:
glucuronoyl esterase, 4-O-methyl-glucuronoyl methylesterase
Source:
Ruminococcus flavefaciens
Molecular Weight:
47,000
Concentration:
Supplied at ~ 370 U/mL
Expression:
Recombinant from Ruminococcus flavefaciens
Specificity:
Glucuronoyl esterase is believed to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin.
Specific Activity:
~ 27 U/mg (40oC, pH 6.5 on pNP-GEUX3)
Unit Definition:
One Unit of glucuronoyl esterase activity is defined as the amount of enzyme required to release one μmole of of p-nitrophenol (pNP) per minute from p-nitrophenyl-22-(methyl 4-O-methyl-α-D-glucopyranosyluronate)-β-D-xylobioside (5 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC. Analysis was carried out using the Glucuronoyl Esterase Assay kit, K-GEUX3.
Temperature Optima:
40oC
pH Optima:
7
Application examples:
Applications in carbohydrate research and in the food industry.
This product has been discontinued (read more).
High purity Glucuronoyl Esterase (Ruminococcus flavefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Safety Information
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