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Isoamylase (Glycogen 6-glucanohydrolase) 
(Flavobacterium odoratum)

Product code: E-ISAMYFO-200U

Content:

€100.00

200 Units

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Content: 200 Units or 800 Units
Shipping Temperature: Ambient
Storage Temperature: 4oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: Isoamylase
EC Number: 3.2.1.68
CAZy Family: GH13
CAS Number: 9067-73-6
Synonyms: isoamylase; glycogen 6-alpha-D-glucanohydrolase
Source: Flavobacterium odoratum
Molecular Weight: 83,000
Concentration: Supplied at ~ 200 U/mL
Expression: Purified from Flavobacterium odoratum
Specificity: Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Specific Activity: ~ 140 U/mg (40oC, pH 6.0 with 1 mM CaClon oyster glycogen)
Unit Definition: One Unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10 mg/mL) in MES buffer (100mM, pH 6.0) plus 1 mM CaCl2 at 40oC.
Temperature Optima: 50oC
pH Optima: 6
Application examples: Applications in carbohydrate research and in the food and feeds, and cereals industry.
Method recognition: AOAC Method 2000.11 and GB Standard 5009.245-2016

Pure Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

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Documents
Certificate of Analysis
Safety Data Sheet
Data Sheet
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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