Guanylate kinase (prokaryote)

Reference code: E-GMPK

500 Units

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

500 Units

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

Minimum 1 year at 4^oC. Check vial for details.

Enzyme Activity:

Kinase

EC Number:

2.7.4.8

CAS Number:

9026-59-9

Synonyms:

guanylate kinase; ATP:(d)GMP phosphotransferase

Source:

Prokaryote

Molecular Weight:

25,756

Concentration:

Supplied at ~ 250 U/mL

Expression:

Recombinant from a Prokaryotic source

Specificity:

Catalyses the reaction:
ATP + GMP = ADP + GDP
Catalyses the ATP-dependent phosphorylation of Guanosine monophosphate (GMP) into Guanosine diphosphate (GDP).

Specific Activity:

~ 50 U/mg (25^oC, pH 7.6 on GMP)

Unit Definition:

One Unit of guanylate kinase is defined as the amount of enzyme required to produce one µmole of GDP from GMP and ATP in the presence of NADH in TEA buffer (73 mM), pH 7.6 at 25^oC.

Temperature Optima:

25^oC

pH Optima:

7.6

Application examples:

Applications in the measurement of GMP.

This product has been discontinued (read more).

High purity recombinant Guanylate kinase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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