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Resistant Starch Assay Kit (Rapid)

00:08   Introduction
01:09    Principle
02:35    Reagent Preparation
06:03    Weighing of samples
06:34    Hydrolysis & Solubilization of Digestible Starch
07:53    Waterbath Alignment
08:22    Ethanol washing & centrifugation
10:26    Procedure
14:26     Measurement of Digestible, Non-Resistant Starch
16:57     Calculations

Resistant Starch Assay Kit (Rapid)
Resistant Starch Assay Kit (Rapid) K-RAPRS
   
Product code: K-RAPRS
€234.00

100 assays per kit

Prices exclude VAT

Available for shipping

Content: 100 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Resistant Starch
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 510
Signal Response: Increase
Linear Range: 4 to 100 mg of D-glucose per assay
Limit of Detection: 0.036 g/100 g
Reaction Time (min): ~ 5 h
Application examples: Plant materials, starch samples and other materials.
Method recognition: An updated modification of: AACC Method 32-40.01, AOAC Method 2002.02 and CODEX Method Type II

Download the K-RAPRS Flyer

The Resistant Starch Assay Kit (Rapid) method is suitable for the measurement of resistant starch in pure starch, cereal and legume seeds and food samples. This method is an update of the method of McCleary et al1 (AOAC Method 2002.02, AACC Method 32-40.01) employing incubation conditions similar to those used in AOAC Method 2017.16 for dietary fiber. The enzyme mixture employed [pancreatic α-amylase (PAA) and amyloglucosidase (AMG)] are those used by Englyst et al.2 except that both enzymes have been purified, standardised and stabilised. Digestion is performed using saturating levels of PAA and AMG with stirring at pH 6 and 37oC for 4 h, to simulate in vivo conditions in the human small intestine. Recent studies on the hydrolysis of “newer” resistant starch materials such as phosphate crosslinked starch (RS4) indicated that these incubation conditions are an essential requirement to obtain meaningful physiologically relevant values for RS.

The incubation conditions parallel those used in AOAC Method 2017.16, a new, rapid integrated procedure for the measurement of total dietary fiber (Megazyme method K-RINTDF). This method is physiologically based and designed to service the definition of DF announced by Codex Alimentarius in 2009.

1. McCleary, B. V., McNally, M. & Rossiter, P. (2002).  Measurement of Resistant Starch by Enzymic Digestion in Starch and Selected Plant Materials - Collaborative Study.  J. AOAC Int., 85, 1103-1111.

2. Englyst, H. N., Kingman, S. M. & Cummings, J. H. (1992).  Classification and measurement of nutritionally important starch fractions.  Eur. J. Clin. Nutr., 46 (Suppl. 2), S33-S50.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet Data Calculator
Safety Information
Symbol : GHS05, GHS08
Signal Word : Danger
Hazard Statements : H314, H315, H319, H334
Precautionary Statements : P260, P261, P264, P280, P284, P301+P330+P331, P302+P352, P303+P361+P353, P304+P340, P501
Safety Data Sheet
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