Pectate Lyase (Aspergillus sp.)

Reference code: E-PCLYAN2

7,500 Units in 3.2 M ammonium sulphate

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

7,500 Units in 3.2 M ammonium sulphate

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

> 4 years at 4^oC

Enzyme Activity:

Pectate Lyase

EC Number:

4.2.2.2

CAZy Family:

PL1

CAS Number:

9015-75-2

Synonyms:

pectate lyase; (1,4)-alpha-D-galacturonan lyase

Source:

Aspergillus sp.

Molecular Weight:

45,000

Concentration:

Supplied at ~ 3,000 U/mL

Expression:

From Aspergillus sp.

Specificity:

Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends.

Specific Activity:

~ 180 U/mg (40^oC, pH 8.0 on polygalacturonic acid)

Unit Definition:

One Unit of pectate lyase activity is defined as the amount of enzyme required to release one µmole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris.HCl buffer (50 mM), pH 8.0 at 40^oC.

Temperature Optima:

40^oC

pH Optima:

Application examples:

Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research.

This product has been discontinued (read more).

High purity Pectate lyase (Aspergillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Publications

Complete Chemical Analysis of Carmagnola Hemp Hurds and Structural Features of Its Components.

Gandolfi, S., Ottolina, G., Riva, S., Fantoni, G. P. & Patel, I. (2013). BioResources, 8(2), 2641-2656.

Complete Chemical Analysis of Carmagnola Hemp Hurds and Structural Features of Its Components.

Gandolfi, S., Ottolina, G., Riva, S., Fantoni, G. P. & Patel, I. (2013). BioResources, 8(2), 2641-2656.

As interest in lignocellulosic biomass as a feedstock for conversion into biofuels is steadily growing, analysis of its components becomes ever more important. The complete chemical composition of waste hemp hurds from the industrial variety "Carmagnola" has been determined to optimize its utilization as a raw material. The results from chemical analysis show that hemp hurds contain 44.0% alpha-cellulose, 25.0% hemicellulose, and 23.0% lignin as major components, along with 4.0% extractives (oil, proteins, amino acids, pectin) and 1.2% ash. Structural and physicochemical properties of hurds components were analysed by FTIR or GC/MS. The data revealed that isolated components are pure and comparable to standard components. Acetone extractives show higher total phenolic content and antioxidant capacity compared with lignin and dichloromethane extractives. Water extractive shows the presence of proteins (1.6%), free amino acids (0.02%), and pectin (0.6%). The degree of esterification of pectin was estimated to be 46.0% by FTIR and enzymatic hydrolysis. The results of this study show that Carmagnola hurds contain low amounts of ash and high amounts of carbohydrates compared with other varieties of hemp hurds; therefore they can be considered as a potential feedstock for biorefinery.

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