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4-Nitrophenyl-β-D-mannopyranoside

4-Nitrophenyl-beta-D-mannopyranoside O-PNPBM
Product code: O-PNPBM
€116.00

100 mg

Prices exclude VAT

Available for shipping

Content: 100 mg
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Physical Form: Powder
Stability: > 10 years under recommended storage conditions
CAS Number: 35599-02-1
Synonyms: p-Nitrophenyl-β-D-mannopyranoside, pNP-β-D-mannopyranoside
Molecular Formula: C12H15NO8
Molecular Weight: 301.3
Purity: > 98%
Substrate For (Enzyme): β-Mannosidase
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400-420

High purity 4-Nitrophenyl-β-D-mannopyranoside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for the measurement of β-mannosidase activity.

See more related colourimetric substrates.

Documents
Certificate of Analysis
Safety Data Sheet
Assay Protocol
Publications
Publication

Global transcriptomic analysis of functional oligosaccharide metabolism in Pediococcus pentosaceus.

Han, D., Shi, R., Yan, Q., Shi, Y., Ma, J. & Jiang, Z. (2021). Applied Microbiology and Biotechnology, 105(4), 1601-1614.

Lactic acid bacteria (LAB) are important in food fermentation and may enhance overall host health. Previous studies to explore LAB metabolism mainly focused on the genera Lacticaseibacillus and Lactococcus. Pediococcus pentosaceus, historically recognized as an important food fermentation bacterial strain, can produce bacteriocins and occasionally demonstrated probiotic functionalities. This study thoroughly surveyed the growth kinetic of three P. pentosaceus isolates in various culture formulations, especially in fructooligosaccharide (FOS), xylooligosaccharide (XOS), or konjac mannooligosaccharide (KMOS) conditions. Results showed that P. pentosaceus effectively metabolized KMOS, the culture of which led to 23.6-fold population increase. However, FOS and XOS were less metabolized by P. pentosaceus. On functional oligosaccharide cultures, P. pentosaceus could result in higher population proliferation, more acidified fermentation environment, and higher glycoside hydrolysis activities in the culture. RNA-Seq analysis classified 1572 out of 1708 putative genes as mRNA-coding genes. The dataset also revealed that the three functional oligosaccharides led to extensive global functional gene regulations. Phosphate conservation and utilization efficiency enhancement may serve as a leading transcriptional regulation direction in functional oligosaccharide metabolisms. In summary, these discovered metabolic characteristics could be employed to support future studies.

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Safety Information
Symbol : GHS07
Signal Word : Warning
Hazard Statements : H315, H319, H335
Precautionary Statements : P261, P264, P271, P280, P302+P352, P304+P340
Safety Data Sheet
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