Content: | 500 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | > 4 years at 4oC |
Enzyme Activity: | Dehydrogenase |
EC Number: | 1.1.1.67 |
CAS Number: | 9001-65-4 |
Synonyms: | mannitol 2-dehydrogenase; D-mannitol:NAD+ 2-oxidoreductase |
Source: | Pseudomona fluorescens |
Molecular Weight: | 56,661 |
Concentration: | Supplied at ~ 300 U/mL |
Expression: | Recombinant from Pseudomona fluorescens |
Specificity: |
Catalyses the reaction: D-Mannitol + NAD+ = D-fructose + NADH + H+ |
Specific Activity: | ~ 50 U/mg (40oC, pH 8.6 on D-mannitol) |
Unit Definition: | One Unit of mannitol dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-mannitol (5.2 mM) in Tris-HCl buffer (64 mM), pH 8.6 at 40oC. |
Temperature Optima: | 40oC |
pH Optima: | 8.6 |
Application examples: | Low Km, suitable for D-mannitol determination. Potential uses in the biotechnology industry. |
This product has been discontinued (read more).
High purity recombinant Mannitol dehydrogenase (Pseudomona fluorescens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
More enzymes for analytical applications available.
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