L-Malate dehydrogenase (Escherichia coli)
Reference code: E-LMDHEC
50,000 Units
This product has been discontinued
Content:
50,000 Units
Shipping Temperature:
Ambient
Storage Temperature:
2-8oC
Formulation:
In 3.2 M ammonium sulphate
Physical Form:
Suspension
Stability:
> 4 years at 4oC
Enzyme Activity:
Dehydrogenase
EC Number:
1.1.1.37
CAS Number:
9001-64-3
Synonyms:
malate dehydrogenase; (S)-malate:NAD+ oxidoreductase
Source:
Escherichia coli
Molecular Weight:
34,501
Concentration:
Supplied at ~ 15,000 U/mL
Expression:
Recombinant from Escherichia coli
Specificity:
Catalyses the reaction:
L-Malate + NAD+ = oxaloacetate + NADH + H+
L-Malate + NAD+ = oxaloacetate + NADH + H+
Specific Activity:
~ 1,700 U/mg (25oC, pH 7.5. on oxaloacetic acid)
Unit Definition:
One Unit of L-malate dehydrogenase is defined as the amount of enzyme required to produce one µmole of NAD+ from NADH in the presence of oxaloacetic acid (0.47 mM) in sodium phosphate buffer (92 mM), pH 7.5 at 25oC.
Temperature Optima:
25oC
pH Optima:
7.5
Application examples:
Applications for the measurement of L-malic acid in the food, fermentation, wine, beverage and dairy industries.
This product has been discontinued (read more).
High purity recombinant L-Malate dehydrogenase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Safety Information
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