Reference code:
E-LMDHEC
50,000 Units
This product has been discontinued
Content: | 50,000 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | > 4 years at 4oC |
Enzyme Activity: | Dehydrogenase |
EC Number: | 1.1.1.37 |
CAS Number: | 9001-64-3 |
Synonyms: | malate dehydrogenase; (S)-malate:NAD+ oxidoreductase |
Source: | Escherichia coli |
Molecular Weight: | 34,501 |
Concentration: | Supplied at ~ 15,000 U/mL |
Expression: | Recombinant from Escherichia coli |
Specificity: |
Catalyses the reaction: L-Malate + NAD+ = oxaloacetate + NADH + H+ |
Specific Activity: | ~ 1,700 U/mg (25oC, pH 7.5. on oxaloacetic acid) |
Unit Definition: | One Unit of L-malate dehydrogenase is defined as the amount of enzyme required to produce one µmole of NAD+ from NADH in the presence of oxaloacetic acid (0.47 mM) in sodium phosphate buffer (92 mM), pH 7.5 at 25oC. |
Temperature Optima: | 25oC |
pH Optima: | 7.5 |
Application examples: | Applications for the measurement of L-malic acid in the food, fermentation, wine, beverage and dairy industries. |
This product has been discontinued (read more).
High purity recombinant L-Malate dehydrogenase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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