Research and White Papers

Megazyme has been contributing to the scientific body of knowledge in cereal chemistry through peer-reviewed research publications since the company’s foundation. These publications contain the fundamental research that has led to the development of a wide range of Megazyme products and should provide keen insight into their subject areas.

In this section:

• White papers

• Peer-reviewed articles on Dietary Fiber and components

• Scientific book chapters on Dietary Fiber
  
  

Reprints of any of the following articles or book chapters can be requested from Megazyme by emailing cs@megazyme.com. You can also find a full list of all Megazyme publications (including those not relevant to Dietary Fiber) in our general Publications section.

White Papers

1. May 2020: AOAC approves revolutionary new method for dietary fiber measurement in foods

Peer Reviewed Articles on Dietary Fiber and Components 

1. McCleary, B. V. & Codd, R. (1991).  Measurement of (1→3),(1→4)-β-D-Glucan in Barley and Oats: A Streamlined Enzymic Procedure.  J. Sci. Food. Agric., 55, 303-312. Link to article
2. McCleary, B. V., Solah, V. & Gibson, T. S. (1994).  Quantitative Measurement of Total Starch in Cereal Flours and Products.  J. Cereal Sci., 20, 51-58. Link to article
3. McCleary, B. V., Gibson, T. S., Solah, V. & Mugford, D. C. (1994).  Total Starch Measurement in Cereal Products: Interlaboratory Evaluation of a Rapid Enzymic Test Procedure.  Cereal Chemistry, 71, 501-505. Link to article 
4. McCleary, B. V. & Mugford, D. C. (1997).  Determination of β-Glucan in Barley and Oats by Streamlined Enzymic Method: Summary of Collaborative Study.  J. AOAC Int., 80, 580-583. Link to publishers website (e-journal not available)
5. Blakeney, A. B., McCleary, B. V. & Mugford, D. C. (1997).  Fructans - Analytical Approaches to a Fibre that Ferments.  Chemistry in Australia, 17-19. Link to article 
6. McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997).  Collaborative evaluation of a simplified assay for total starch in cereal products (AACC Method 76-13).  Cereal Foods World, 42, 476-480. Link to article
7. McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997).  Measurement of Total Starch in Cereal Products by Amyloglucosidase-α-Amylase Method: Collaborative Study.  J. AOAC Int., 80, 571-579. Link to publishers website (e-journal not available)
8. McCleary, B. V. & Blakeney, A. B. (1999).  Measurement of Inulin and Oligofructan.  Cereal Foods World, 44, 398-406. Link to article
9. McCleary, B. V. (1999).  Measuring Dietary Fibre.  The World of Ingredients, 50-53. Link to article
10. McCleary, B. V. (1999).  Enzyme Purity and Activity in Fibre Determinations.  Cereal Foods World, 44, 590-596. Link to article 
11. McCleary, B. V., Murphy, A. & Mugford, D. C. (2000).  Measurement of Total Fructan in Foods by Enzymatic/Spectrophotometric Method: Collaborative study.  J. AOAC Int., 83, 356-364. Link to article
12. McCleary, B. V. (2000).  Importance of Enzyme Purity and Activity in the Measurement of Total Dietary Fibre and Dietary Fibre Components.  J. AOAC Int., 83, 997-1005. Link to article
13. McCleary, B. V. (2001).  Two Issues in Dietary Fiber Measurement.  Cereal Foods World, 46, 164-165. Link to article
14. McCleary, B. V. & Monaghan, D. A. (2002).  Measurement of Resistant Starch.  J. AOAC Int., 85, 665-675. Link to article
15. McCleary, B. V., McNally, M. & Rossiter, P. (2002).  Measurement of Resistant Starch by Enzymic Digestion in Starch and Selected Plant Materials: Collaborative Study.  J. AOAC Int., 85, 1103-1111. Link to article
16. McCleary, B. V. (2003).  Dietary Fibre Analysis.  Proceedings of the Nutrition Society, 62, 3-9. Link to article
17. McCleary, B. V. & Rossiter, P. (2004).  Measurement of Novel Dietary Fibres.  J. AOAC Int., 87, 707-717. Link to article
18. McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006).  Measurement of Carbohydrates in Grain, Feed and Food.  J. Sci. Food. Agric., 86, 1648-1661. Link to article
19. McCleary, B. V. (2007).  An Integrated Procedure for the Measurement of Total Dietary Fibre (including resistant starch), non-digestible oligosaccharides and available carbohydrates. Analytical and Bioanalytical Chemistry, 389, 291-308. Link to article
20. McCleary, B. V., Mills, C. & Draga, A. (2009).  Development and Evaluation of an Integrated Method for the Measurement of Total Dietary Fibre.  Quality Assurance and Safety of Crops & Foods Special Issue: Dietary Fibre, 4, 213-224. Link to article
21. McCleary, B. V., DeVries, J. W., Rader, J. I., Cohen, G., Prosky, P., Mugford, D. C., Champ, M. & Okuma, K. (2010).  Determination of Total Dietary Fiber (CODEX Definition) by Enzymatic-Gravimetric Method and Liquid Chromatography: Collaborative Study.  J. AOAC Int., 93, 221-233. Link to article
22. McCleary, B. V. (2010).  Development of an Integrated Total Dietary Fiber Method Consistent with the Codex Alimentarius Definition.  Cereal Foods World, 55(1), 24-28. Link to publishers website (e-journal not available)
23.  McCleary, B. V., DeVries, J. W., Rader, J. I., Cohen, G., Prosky, P., Mugford, D. C., Champ, M. & Okuma, K. (2012).  Collaborative Study Report: Determination of Insoluble, Soluble, and Total Dietary Fiber (Codex Definition) by an Enzymatic-Gravimetric Method and Liquid Chromatography.  J. AOAC Int., 95, 824-844. Link to article
24. McCleary, B. V., Sloane, N., Draga, A. & Lazewska, I. (2013).  Measurement of Total Dietary Fiber Using AOAC Method 2009.01 (AACC International Approved Method 32-45.01): Evaluation and Updates.  Cereal Chemistry, 90(4), 396-414. Link to article
25. McCleary, B. V. (2014).  Modification to AOAC Official Methods 2009.01 and 2011.25 to Allow for Minor Overestimation of Low Molecular Weight Soluble Dietary Fiber in Samples Containing Starch.  J. AOAC Int., 97(3), 896-901. Link to article
26. McCleary, B. V., Sloane, N. & Draga, A (2015).  Determination of total dietary fibre and available carbohydrates: A rapid integrated procedure that simulates in vivo digestion.  Starch/Stärke, 66, 1-24. Link to article
27. McCleary, B. V., Cox, J. & McKie, V. A. (2018).  Collaborative Study on Determination of Total Dietary Fiber (Digestion-Resistant Carbohydrates per Codex Definition) by Rapid Enzymatic-Gravimetric Method and Liquid Chromatography.  Cereal Foods World, 63(2), 80-84. Link to article

Book Chapters on Dietary Fiber

1. McCleary, B. V. (1998).  Measurement of Inulin and Inulin-Degrading Enzymes. “Proceedings of the Seventh Seminar on Inulin”, (A. Fuchs and A. Van Laere, Eds.), European Fructan Association, pp. 36-45. Link to Book
2. Brooks, M., Campbell, R. & McCleary, B. V. (2001).  Novel Galactomannans and Galacto-manno Oligosaccharides from Guar. “Advanced Dietary Fibre Technology”, (B. V. McCleary and L. Prosky, Eds.), Blackwell Science, Oxford, U.K., pp. 331-344. Link to book chapter
3. McCleary, B. V. (2001).  Measurement of Dietary Fibre Components: the Importance of Enzyme Purity, Activity and Specificity. “Advanced Dietary Fibre Technology”, (B. V. McCleary and L. Prosky, Eds.), Blackwell Science, Oxford, U.K., pp. 89-105. Link to book
4. McCleary, B. V. & Rossiter, P. C. (2007).  Dietary Fiber and Available Carbohydrates. “Dietary Fiber: An International Perspective for Harmonization of Health Benefits and Energy Values”, (Dennis T. Gordon and Toshinao Goda, Eds.), AACC International, Inc., pp. 31-59. Link to book
5. AOAC Official Method 2011.25. (2012).  Insoluble, soluble, and total dietary fiber in foods. “The Official Methods of Analysis SM, 19th Edition”, (G. W. Latimer, Ed.). Link to book chapter
6. McCleary, B. V., Draga, A. & Sloane, N. (2013).  Dietary fibre analysis in foods. “Fibre-rich and wholegrain foods”, (Jan A. Declour and Kaisa Poutanen, Eds.), Woodhead Publishing Limited, pp. 25-61. Link to book chapter
7. McCleary, B. V. (2013). Measurement of resistant starch and incorporation of resistant starch into dietary fibre measurements. “Resistant Starch Sources, Applications and Health Benefits”, (Yong-Cheng Shi and Clodualdo C. Maningat, Eds.), John Wiley and Sons Ltd, pp. 131-144. Link to book