exo-α-Sialidase (Clostridium perfringens)

Reference code: E-SIALCP

50 Units

This product has been discontinued

Buy on Neogen.com

Share by email Ask a Scientist

Data Sheet

SDS

Certificate of Analysis

Content:

50 Units

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 20 mM tris buffer containing 50 mM NaCl₂ and 5 mM EDTA

Physical Form:

Suspension

Stability:

> 6 months under recommended storage conditions

Enzyme Activity:

α-Sialidase

EC Number:

3.2.1.18

CAZy Family:

GH33

CAS Number:

9001-67-6

Synonyms:

exo-alpha-sialidase; acetylneuraminyl hydrolase

Source:

Clostridium perfringens

Molecular Weight:

43,600

Concentration:

Supplied at ~ 500 U/mL

Expression:

Recombinant from Clostridium perfringens

Specificity:

Hydrolysis of unbranched, non-reducing terminal α-2,3-linked, α-2,6-linked >> α-2,8-linked N-acetylneuraminic acid (NANA; Neu5Ac) residues from glycoproteins and oligosaccharides of glycoconjugates.

Specific Activity:

~ 300 U/mg (37^oC, pH 7.0 on pNP-α-D-N-acetylneuraminic acid)

Unit Definition:

One Unit of sialidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-α-D-N-acetylneuraminic acid (1 mM) in sodium phosphate buffer (100 mM), pH 7.0 at 37^oC.

Temperature Optima:

37^oC

pH Optima:

Application examples:

Applications in clinical chemistry, glycobiology and carbohydrate research.

This product has been discontinued.

High purity recombinant exo-α-sialidase (Clostridium perfringens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Explore more CAZy enzymes and purified glycobiology enzyme products available.