|Formulation:||In 3.2 M ammonium sulphate|
|Stability:||Minimum 1 year at 4oC. Check vial for details.|
|Enzyme Activity:||Other Activities|
|Synonyms:||succinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming)|
|Concentration:||Supplied at ~ 220 U/mL|
|Expression:||Recombinant from a Prokaryotic source|
Catalyses the reaction:
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
|Specific Activity:||~ 8 U/mg (25oC, pH 8.4 on succinic acid)|
|Unit Definition:||One Unit of Succinyl-CoA synthetase activity is defined as the amount of enzyme required to release one µmole of succinyl CoA from succinic acid (5.8 mM) per minute in the presence of NADH and Coenzyme A in Glycylglycine buffer (34 mM), pH 8.4 at 25oC.|
|Application examples:||Applications for the measurement of succinic acid in the clinical chemistry, food, wine and dairy industries.|
High purity recombinant Succinyl-CoA synthetase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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