Succinyl-CoA synthetase (prokaryote)

Reference code: E-SCOAS

550 Units

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

550 Units

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

> 1 year under recommended storage conditions

Enzyme Activity:

Other Activities

EC Number:

6.2.1.5

CAS Number:

9080-33-5

Synonyms:

succinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming)

Source:

Prokaryote

Molecular Weight:

30,843,
41,393

Concentration:

Supplied at ~ 220 U/mL

Expression:

Recombinant from a Prokaryotic source

Specificity:

Catalyses the reaction:
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA

Specific Activity:

~ 9 U/mg (25^oC, pH 8.4 on succinic acid)

Unit Definition:

One Unit of Succinyl-CoA synthetase activity is defined as the amount of enzyme required to release one µmole of succinyl CoA from succinic acid (5.8 mM) per minute in the presence of NADH and Coenzyme A in Glycylglycine buffer (34 mM), pH 8.4 at 25^oC.

Temperature Optima:

25^oC

pH Optima:

8.4

Application examples:

Applications for the measurement of succinic acid in the clinical chemistry, food, wine and dairy industries.

This product has been discontinued.

High purity recombinant Succinyl-CoA synthetase (prokaryote) for use in research, biochemical enzyme assays and analytical testing applications.

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