β-N-Acetylhexosaminidase (prokaryote)

Reference code: E-BNAHP

100 Units at 40^oC

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

100 Units at 40^oC

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

Minimum 1 year at 4^oC. Check vial for details.

Enzyme Activity:

β-N-Acetyl-hexosaminidase

EC Number:

3.2.1.52

CAZy Family:

GH20

CAS Number:

9012-33-3

Synonyms:

beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase

Source:

Prokaryote

Molecular Weight:

52,000

Concentration:

Supplied at ~ 1,000 U/mL

Expression:

Recombinant from a Prokaryote

Specificity:

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-β-D-hexosaminides from glycoproteins and oligosaccharides.

Specific Activity:

~ 260 U/mg protein (40^oC, pH 4.0 on pNP-β-D-N-acetylglucosamine);
~ 723 U/mg protein (60^oC, pH 4.0 on pNP-β-D-N-acetylglucosamine)

Unit Definition:

One Unit of β-N-acetyl-D-hexosaminidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-β-D-N-acetylglucosamine (1 mM) in citrate-phosphate buffer (100 mM), pH 4.0 at the mentioned temperatures.

Temperature Optima:

60^oC

pH Optima:

Application examples:

For use in glycobiology research.

This product has been discontinued (read more).

High purity β-N-Acetylhexosaminidase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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