Dextranase (Chaetomium sp.)

Reference code: E-DEXT

320,000 Units at 40^oC

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

320,000 Units at 40^oC

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 50% (v/v) glycerol

Physical Form:

Solution

Stability:

> 4 years at 4^oC

Enzyme Activity:

endo-Dextranase

EC Number:

3.2.1.11

CAZy Family:

Other, Non-classified sequences

CAS Number:

9025-70-1

Synonyms:

endo-dextranase; α-D-1,6-glucan-6-glucanohydrolase; 1,6-α-D-glucan 6-glucanohydrolase

Source:

Chaetomium sp.

Molecular Weight:

66,000

Concentration:

Supplied at ~ 8,000 U/mL

Expression:

Purified from Chaetomium sp.

Specificity:

Hydrolysis of α-1,6 glucosidic linkages in dextran.

Specific Activity:

~ 490 U/mg protein at pH 5.0 and 40^oC;
~ 607 U/mg protein at pH 5.0 and 50^oC

Unit Definition:

One Unit of dextranase activity is defined as the amount of enzyme required to release one µmole of reducing-sugar equivalents from Dextran B512 per minute under the conditions stated.

Temperature Optima:

60^oC

pH Optima:

Application examples:

For use in research.

This product has been discontinued (read more).

High purity Dextranase (Chaetomium sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

See entire list of Carbohydrate Active enZYmes.

Publications

Detailed structural characterization of glucans produced by glucansucrases from Leuconostoccitreum TMW 2.1194.

Münkel, F., Bechtner, J., Eckel, V., Fischer, A., Herbi, F., Jakob, F. & Wefers, D. (2019). Journal of Agricultural and Food Chemistry, 67(24), 6856-6866.

Detailed structural characterization of glucans produced by glucansucrases from Leuconostoccitreum TMW 2.1194.

Münkel, F., Bechtner, J., Eckel, V., Fischer, A., Herbi, F., Jakob, F. & Wefers, D. (2019). Journal of Agricultural and Food Chemistry, 67(24), 6856-6866.

The water kefir organism Leuconostoc citreum TMW 2.1194 forms highly branched dextrans with O3- and O4-bound side chains. To obtain detailed information on the enzymatic synthesis of these polymers, the four glucansucrases encoded by Leuconostoc citreum TMW 2.1194 were cloned, heterologously expressed, and used for polysaccharide production. Molecular and macromolecular structure of the synthesized glucans were analyzed by methylation analysis, two-dimensional NMR spectroscopy, oligosaccharide analysis after partial hydrolysis, and asymmetric flow field-flow fractionation. It was demonstrated that two glucansucrases form insoluble glucans with variously branched dextran sections and varying portions of consecutive, 1,3-linked glucose units. In contrast, the other two glucansucrases synthesized O3-(Lc6255) and O4-branched (Lc1785) soluble dextrans. Analysis, isolation, and characterization of enzymatically liberated oligosaccharides showed that monomeric and elongated side chains are abundant in both polysaccharides. From the structures and size distributions it was concluded that Lc1785 is mainly responsible for synthesis of fermentatively produced soluble dextrans.

Link to Article