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α-L-Arabinofuranosidase (Ustilago maydis)

alpha-L-Arabinofuranosidase Ustilago maydis E-ABFUM
Product code: E-ABFUM
€152.00

400 Units on WAX at 40oC

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Content: 400 Units on WAX at 40oC
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: α-Arabinofuranosidase
EC Number: 3.2.1.55
CAZy Family: GH62
CAS Number: 9067-74-7
Synonyms: non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase
Source: Ustilago maydis
Molecular Weight: 35,000
Concentration: Supplied at ~ 200 U/mL
Expression: Recombinant from Ustilago maydis
Specificity: Hydrolysis of terminal, non-reducing α-L-arabinofuranose from singly substituted xylose residues in arabinoxylan (α-1,2 > α-1,3).  Does not hydrolyse α-L-arabinofuranose from doubly substituted xylose residues in arabinoxylan.
Specific Activity: ~ 9 U/mg protein (40oC, pH 5.0 on wheat arabinoxylan)
Unit Definition: One Unit of α-L-arabinfuranosidase activity is defined as the amount of enzyme required to release one µmole of arabinose per minute from wheat arabinoxylan (10 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40oC.
Temperature Optima: 40oC
pH Optima: 5
Application examples: For use in plant cell wall carbohydrate and biofuels research.

High purity α-L-Arabinofuranosidase (Ustilago maydis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Highly active on wheat arabonoxylan.

We offer related Carbohydrate Active enZYmes.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Megazyme publication
Hydrolysis of wheat flour arabinoxylan, acid-debranched wheat flour arabinoxylan and arabino-xylo-oligosaccharides by β-xylanase, α-L-arabinofuranosidase and β-xylosidase.

McCleary, B. V., McKie, V. A., Draga, A., Rooney, E., Mangan, D. & Larkin, J. (2015). Carbohydrate Research, 407, 79-96.

A range of α-L-arabinofuranosyl-(1-4)-β-D-xylo-oligosaccharides (AXOS) were produced by hydrolysis of wheat flour arabinoxylan (WAX) and acid debranched arabinoxylan (ADWAX), in the presence and absence of an AXH-d3 α-L-arabinofuranosidase, by several GH10 and GH11 β-xylanases. The structures of the oligosaccharides were characterised by GC-MS and NMR and by hydrolysis by a range of α-L-arabinofuranosidases and β-xylosidase. The AXOS were purified and used to characterise the action patterns of the specific α-L-arabinofuranosidases. These enzymes, in combination with either Cellvibrio mixtus or Neocallimastix patriciarum β -xylanase, were used to produce elevated levels of specific AXOS on hydrolysis of WAX, such as 32-α-L-Araf-(1-4)-β-D-xylobiose (A3X), 23-α-L-Araf-(1-4)-β-D-xylotriose (A2XX), 33-α-L-Araf-(1-4)-β-D-xylotriose (A3XX), 22-α-L-Araf-(1-4)-β-D-xylotriose (XA2X), 32-α-L-Araf (1-4)-β-D-xylotriose (XA3X), 23-α-L-Araf-(1-4)-β-D-xylotetraose (XA2XX), 33-α-L-Araf-(1-4)-β-D-xylotetraose (XA3XX), 23 ,33-di-α-L-Araf-(1-4)-β-D-xylotriose (A2+3XX), 23,33-di-α-L-Araf-(1-4)-β-D-xylotetraose (XA2+3XX), 24,34-di-α-L-Araf-(1-4)-β-D-xylopentaose (XA2+3XXX) and 33,34-di-α-L-Araf-(1-4)-β-D-xylopentaose (XA3A3XX), many of which have not previously been produced in sufficient quantities to allow their use as substrates in further enzymic studies. For A2,3XX, yields of approximately 16% of the starting material (wheat arabinoxylan) have been achieved. Mixtures of the α-L-arabinofuranosidases, with specific action on AXOS, have been combined with β-xylosidase and β-xylanase to obtain an optimal mixture for hydrolysis of arabinoxylan to L-arabinose and D-xylose.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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