4-Nitrophenyl-β-xylotrioside
20 mg
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| Content: | 20 mg |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Physical Form: | Solid |
| Stability: | > 10 years under recommended storage conditions |
| CAS Number: | 173468-29-6 |
| Molecular Formula: | C21H29NO15 |
| Molecular Weight: | 535.4 |
| Purity: | > 98% |
| Substrate For (Enzyme): | endo-1,4-β-Xylanase |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400-420 |
High purity 4-Nitrophenyl-β-xylotrioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
A substrate for research into xylanase (endo-1,4-β-xylanase) or xylose degrading enzymes.
Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.
endo-1,4-β-Xylanase (EC 3.2.1.8) is employed across a broad range of industries including animal feed, brewing, baking, biofuels, detergents and pulp (paper). Despite its importance, a rapid, reliable, reproducible, automatable assay for this enzyme that is based on the use of a chemically defined substrate has not been described to date. Reported herein is a new enzyme coupled assay procedure, termed the XylX6 assay, that employs a novel substrate, namely 4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-45-O-glucosyl-xylopentaoside. The development of the substrate and associated assay is discussed here and the relationship between the activity values obtained with the XylX6 assay versus traditional reducing sugar assays and its specificity and reproducibility were thoroughly investigated.
Hide Abstract| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
