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4-Nitrophenyl-β-xylotrioside

4-Nitrophenyl-beta-xylotrioside O-PNPX3
Product code: O-PNPX3
€274.00

20 mg

Prices exclude VAT

Available for shipping

Content: 20 mg
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Physical Form: Solid
Stability: > 10 years under recommended storage conditions
CAS Number: 173468-29-6
Molecular Formula: C21H29NO15
Molecular Weight: 535.4
Purity: > 98%
Substrate For (Enzyme): endo-1,4-β-Xylanase
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400-420

High purity 4-Nitrophenyl-β-xylotrioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

A substrate for research into xylanase (endo-1,4-β-xylanase) or xylose degrading enzymes.

Documents
Certificate of Analysis
Safety Data Sheet
Booklet
Publications
Megazyme publication
Novel substrates for the automated and manual assay of endo-1,4-β-xylanase.

Mangan, D., Cornaggia, C., Liadova, A., McCormack, N., Ivory, R., McKie, V. A., Ormerod, A. & McCleary, D. V. (2017). Carbohydrate Research, 445, 14-22.

endo-1,4-β-Xylanase (EC 3.2.1.8) is employed across a broad range of industries including animal feed, brewing, baking, biofuels, detergents and pulp (paper). Despite its importance, a rapid, reliable, reproducible, automatable assay for this enzyme that is based on the use of a chemically defined substrate has not been described to date. Reported herein is a new enzyme coupled assay procedure, termed the XylX6 assay, that employs a novel substrate, namely 4,6-O-(3-ketobutylidene)-4-nitrophenyl-β-45-O-glucosyl-xylopentaoside. The development of the substrate and associated assay is discussed here and the relationship between the activity values obtained with the XylX6 assay versus traditional reducing sugar assays and its specificity and reproducibility were thoroughly investigated.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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