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3-Hydroxybutyrate dehydrogenase (prokaryote)

Product code: E-HBDH

200 Units

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Content: 200 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: Dehydrogenase
EC Number:
CAS Number: 9028-38-0
Synonyms: 3-hydroxybutyrate dehydrogenase; (R)-3-hydroxybutanoate:NAD+ oxidoreductase
Source: Prokaryote
Molecular Weight: 29,177
Concentration: Supplied at ~ 100 U/mL
Expression: Recombinant from a Prokaryotic source
Specificity: Catalyses the reaction:
D-3-Hydroxybutyrate + NAD+ = acetoacetate + NADH + H+
Specific Activity: ~ 77.4 U/mg (25oC, pH 8.0 on D-β-hydroxybutyric acid)
Unit Definition: One Unit of 3-hydroxybutyrate dehydrogenase is defined as the amount of enzyme required to oxidise one µmole of D-β-hydroxybutyric acid (28.3 mM) per minute in the presence of NAD+ in Tris-HCl buffer (143 mM), pH 8.0 at 25oC.
Temperature Optima: 25oC
pH Optima: 8
Application examples: Applications for the measurement of D-3-Hydroxybutyrate in the food, fermentation, wine, beverage and dairy industries.

High purity recombinant 3-Hydroxybutyrate dehydrogenase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Certificate of Analysis
Safety Data Sheet
Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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