3-Hydroxybutyrate dehydrogenase (prokaryote)

Reference code: E-HBDH

200 Units

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

200 Units

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

Minimum 1 year at 4^oC. Check vial for details.

Enzyme Activity:

Dehydrogenase

EC Number:

1.1.1.30

CAS Number:

9028-38-0

Synonyms:

3-hydroxybutyrate dehydrogenase; (R)-3-hydroxybutanoate:NAD⁺ oxidoreductase

Source:

Prokaryote

Molecular Weight:

29,177

Concentration:

Supplied at ~ 100 U/mL

Expression:

Recombinant from a Prokaryotic source

Specificity:

Catalyses the reaction:
D-3-Hydroxybutyrate + NAD⁺ = acetoacetate + NADH + H⁺

Specific Activity:

~ 77.4 U/mg (25^oC, pH 8.0 on D-β-hydroxybutyric acid)

Unit Definition:

One Unit of 3-hydroxybutyrate dehydrogenase is defined as the amount of enzyme required to oxidise one µmole of D-β-hydroxybutyric acid (28.3 mM) per minute in the presence of NAD⁺ in Tris-HCl buffer (143 mM), pH 8.0 at 25^oC.

Temperature Optima:

25^oC

pH Optima:

Application examples:

Applications for the measurement of D-3-Hydroxybutyrate in the food, fermentation, wine, beverage and dairy industries.

This product has been discontinued (read more).

High purity recombinant 3-Hydroxybutyrate dehydrogenase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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