3-Hydroxybutyrate dehydrogenase (prokaryote)
Reference code: E-HBDH
200 Units
This product has been discontinued
Content:
200 Units
Shipping Temperature:
Ambient
Storage Temperature:
2-8oC
Formulation:
In 3.2 M ammonium sulphate
Physical Form:
Suspension
Stability:
Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity:
Dehydrogenase
EC Number:
1.1.1.30
CAS Number:
9028-38-0
Synonyms:
3-hydroxybutyrate dehydrogenase; (R)-3-hydroxybutanoate:NAD+ oxidoreductase
Source:
Prokaryote
Molecular Weight:
29,177
Concentration:
Supplied at ~ 100 U/mL
Expression:
Recombinant from a Prokaryotic source
Specificity:
Catalyses the reaction:
D-3-Hydroxybutyrate + NAD+ = acetoacetate + NADH + H+
D-3-Hydroxybutyrate + NAD+ = acetoacetate + NADH + H+
Specific Activity:
~ 77.4 U/mg (25oC, pH 8.0 on D-β-hydroxybutyric acid)
Unit Definition:
One Unit of 3-hydroxybutyrate dehydrogenase is defined as the amount of enzyme required to oxidise one µmole of D-β-hydroxybutyric acid (28.3 mM) per minute in the presence of NAD+ in Tris-HCl buffer (143 mM), pH 8.0 at 25oC.
Temperature Optima:
25oC
pH Optima:
8
Application examples:
Applications for the measurement of D-3-Hydroxybutyrate in the food, fermentation, wine, beverage and dairy industries.
This product has been discontinued (read more).
High purity recombinant 3-Hydroxybutyrate dehydrogenase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Safety Information
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