myo-Inositol dehydrogenase (Bacillus subtilis)

Reference code: E-INDHBS

500 Units

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

500 Units

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

Minimum 1 year at 4^oC. Check vial for details.

Enzyme Activity:

Dehydrogenase

EC Number:

1.1.1.18

CAS Number:

9028-25-5

Synonyms:

inositol 2-dehydrogenase; myo-Inositol:NAD⁺ 2-oxidoreductase

Source:

Bacillus subtilis

Molecular Weight:

39,417

Concentration:

Supplied at ~ 250 U/mL

Expression:

Recombinant from Bacillus Subtilis

Specificity:

Catalyses the reaction:
myo-inositol + NAD⁺ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H⁺

Specific Activity:

~ 50 U/mg (25^oC, pH 9.6 on myo-inositol)

Unit Definition:

One Unit of myo-inositol dehydrogenase activity is defined as the amount of enzyme required to produce one µmole of scyllo-inosose and NADH from myo-inositol (123 mM) and NAD⁺ per minute in Glycylglycine buffer (492mM), pH 9.6 at 25^oC.

Temperature Optima:

25^oC

pH Optima:

9.6

Application examples:

Applications for the measurement of myo-inositol acid in the food, fermentation, wine, beverage and dairy industries.

This product has been discontinued (read more).

High purity recombinant myo-Inositol dehydrogenase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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