Analytical Method Videos: Dietary Fiber/Starch

00:05  Introduction
01:16  Principle
03:28  Reagent Preparation
05:56  Weighing of Samples & Buffer Addition
07:26  Hydrolysis of Digestible Starch Method A
07:51  Hydrolysis of Digestible Starch Method B
10:43  Procedure
16:23  Calculations

00:05  Introduction
01:42  Principle
04:11  Reagent Preparation
07:54  Enzyme Digestion: Method 1 - Analysing ~ 0.5 g of sample
09:21  Method 1 (A) – Addition of PAA + AMG solution
09:50  Method 1 (B) - Addition of PAA + AMG suspension
10:23  Enzyme Digestion: Method 2 - Analysing ~ 0.1 g of sample
11:34  Method 2 (A) – Addition of PAA + AMG solution
12:03  Method 2 (B) - Addition of PAA + AMG suspension
12:33  Preparation of Stopping Reagents
13:12  Determination of Digestible Starch
18:27  Determination of Resistant Starch
25:53  Calculations

00:00  Principle of the Assay
02:12  Kit Contents
02:40  Reagent Preparation
07:05  Weighing of Samples, Addition of Buffer plus Amylase & AMG
09:03  Addition of Tris Base and Inactivation of Amylase & AMG
10:12  Incubation with Protease
11:12  A. Determination of HMWDF & LMWSDF
11:16  (i): pH Adjustment & Addition of D-Sorbitol & Ethanol
13:20  (ii): Filtration & Recovery of HMWDF & LMWSDF
17:56  (iii): Ash Determination
18:13  (iv): Protein Determination
19:12  (v): Evaporation of Aqueous Ethanol & Recovery of LMWSDF
21:49  (vi): Desalting of Samples with Ion Exchange Resin
26:16  (vii): Liquid Chromatography of Desalted Samples
27:09  B. Determination of IDF, HMWDF & LMWSDF
29:07  (i): pH Adjustment of the Incubation Mixture and Addition of D-Sorbitol
31:10  (ii): Filtration and Recovery of IDF, HMWDF & LMWSDF
36:02  Calculation of Dietary Fiber Content

00:05  Introduction
06:46  Principle
06:46  Reagent Preparation
Procedure
09:45  Weighing of samples
10:13  Incubation with PAA and AMG
11:27  Preparation of Reagent Blanks
Method A – Measurement of TDF as HMWDF + SDFS
11:48  pH Adjustment & protease treatment
13:59  Alcohol Precipitation of HMWSDF
14:36  Filtration and separation of HMWDF (IDF + SDFP) and SDFS
17:21  Aa. Determination of HMWDF (IDF + SDFP)
18:31  Ab. Determination of SDFS
18:35  (i) Recovery, sub-sampling and concentration of SDFS
22:29  (ii) Chromatography of samples on HPLC
23:50  Calculations of TDF – as HMWDF (IDF + SDFP) plus SDFS
Method B – Measurement of TDF as IDF + SDFP + SDFS
27:06  Digestion of samples
27:50  Separation of IDF from SDFP & SDFS
29:47  Ba. Determination of IDF
31:15  Precipitation and recovery of SDFP
32:00  Bb. Determination of SDFP
32:21  Bc. Determination of SDFS
32:48  Calculations – TDF as IDF plus SDFP plus SDFS

00:08   Introduction
01:09  Principle
02:25  Reagent Preparation
06:03  Weighing of samples
06:34  Hydrolysis & Solubilization of Digestible Starch
07:53  Waterbath Alignment
08:22  Ethanol washing & centrifugation
10:26  Procedure
14:26  Measurement of Digestible, Non-Resistant Starch
16:57  Calculations

00:04   Introduction
01:24  Principle
02:22  Reagent Preparation
04:58  Weighing of Samples
05:51  Addition of α-amylase
07:07  Addition of amyloglucosidase
07:49  Glucose determination
09:16  Calculations
11:19  Further information

00:06  Introduction
02:31  Principle
04:27  Preparation of Fritted Crucibles
04:44  Sample Preparation
05:43  Reagent Preparation
06:12  Weighing of Samples
06:35  Incubation with heat stable α-amylase
08:24  Incubation with Protease
09:35  Incubation with Amyloglucosidase
10:18  Method A – Measurement of TDF as HMWDF
14:44  Method B – Separation of TDF components into IDF and SDFP
14:48  Measurement of IDF
18:01  Precipitation & Recovery of SDFP component
19:19  Calculations

00:03   Introduction
02:16  Principle
03:34  Preparation of Fritted Crucibles
03:52  Sample Preparation
04:50  Reagent Preparation
05:19  Weighing of Samples
05:44  Incubation with heat stable α-amylase
07:15  Incubation with Protease
08:11  Incubation with Amyloglucosidase
09:18  Precipitation of High Molecular Weight Soluble Dietary Fiber
09:59  Filtration & Washing of Dietary Fiber (HMWDF)
12:54  Determination of “Total” Dietary Fiber (HMWDF)
14:13  Calculations

00:05  Introduction
02:11  Principle
03:26  Reagent and Sample Preparation
06:22  RTS Method (Solubilization & Hydrolysis of Starch)
06:22  RTS-NaOH Method (Solubilization & Hydrolysis of Starch)
15:41  Calculations