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D-Fructose/D-Glucose Assay Kit

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00:08  Introduction
01:05   Principle
02:29   Reagent Preparation
03:06   Procedure
06:41    Calculations

D-Fructose D-Glucose Assay Kit K-FRUGL Scheme
Product code: K-FRUGL

110 assays (manual) / 1100 assays (microplate) / 1100 assays (auto-analyser)

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Content: 110 assays (manual) / 1100 assays (microplate) / 1100 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: D-Fructose, D-Glucose
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Increase
Linear Range: 4 to 80 µg of D-glucose, D-fructose or sucrose per assay
Limit of Detection: 0.66 mg/L
Reaction Time (min): ~ 13 min
Application examples: Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread, bakery products, candies, desserts, confectionery, ice-cream, fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals, paper and other materials (e.g. biological cultures, samples, etc.).
Method recognition: Methods based on this principle have been accepted by AOAC Method 985.09, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC

D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuantTM  Wave Spectrophotometer (D-MQWAVE).

See more of our monosaccharide assay kits.

Scheme-K-FRUGL FRUGL Megazyme

  • Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
  • PVP incorporated to prevent tannin inhibition 
  • Validated by the University of Wine, Suze la Rousse, France 
  • Very competitive price (cost per test) 
  • All reagents stable for > 2 years after preparation (manual analysis applications) 
  • Rapid reaction at either 25 or 37o
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats
Validation of Methods
Certificate of Analysis
Safety Data Sheet
FAQs Assay Protocol Data Calculator Product Performance Validation Report
Megazyme publication

Megazyme “advanced” wine test kits general characteristics and validation.

Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.

Many of the enzymatic test kits are official methods of prestigious organisations such as the Association of Official Analytical Chemicals (AOAC) and the American Association of Cereal Chemists (AACC) in response to the interest from oenologists. Megazyme decided to use its long history of enzymatic bio-analysis to make a significant contribution to the wine industry, by the development of a range of advanced enzymatic test kits. This task has now been successfully completed through the strategic and comprehensive process of identifying limitations of existing enzymatic bio-analysis test kits where they occurred, and then using advanced techniques, such as molecular biology (photo 1), to rapidly overcome them. Novel test kits have also been developed for analytes of emerging interest to the oenologist, such as yeast available nitrogen (YAN; see pages 2-3 of issue 117 article), or where previously enzymes were simply either not available, or were too expensive to employ, such as for D-mannitol analysis.

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Megazyme publication

Grape and wine analysis: Oenologists to exploit advanced test kits.

Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.

It is without doubt that testing plays a pivotal role throughout the whole of the vinification process. To produce the best possible quality wine and to minimise process problems such as “stuck” fermentation or troublesome infections, it is now recognised that if possible testing should begin prior to harvesting of the grapes and continue through to bottling. Traditional methods of wine analysis are often expensive, time consuming, require either elaborate equipment or specialist expertise and frequently lack accuracy. However, enzymatic bio-analysis enables the accurate measurement of the vast majority of analytes of interest to the wine maker, using just one piece of apparatus, the spectrophotometer (see previous issue No. 116 for a detailed technical review). Grape juice and wine are amenable to enzymatic testing as being liquids they are homogenous, easy to manipulate, and can generally be analysed without any sample preparation.

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Scheffersomyces stipitis ability to valorize different residual biomasses for vitamin B9 production.

Mastella, L., Senatore, V., Beltrani, T. & Branduardi, P. (2022). Microbial Biotechnology.

Sugar beet pulp (SBP), sugar beet molasses (SBM) and unfermented grape marcs (UGM) represent important waste in the agro-food sector. If suitably pre-treated, hexose and pentose sugars can be released in high quantities and can subsequently be used by appropriate cell factories as growth media and for the production of (complex) biomolecules, accomplishing the growing demand for products obtained from sustainable resources. One example is vitamin B9 or folate, a B-complex vitamin currently produced by chemical synthesis, almost exclusively in the oxidized form of folic acid (FA). It is therefore desirable to develop novel competitive strategies for replacing its current fossil-based production with a sustainable bio-based process. In this study, we assessed the production of natural folate by the yeast Scheffersomyces stipitis, investigating SBM, SBP and UGM as potential growth media. Pre-treatment of SBM and SBP had previously been optimized in our laboratory; thus, here we focused only on UGM pre-treatment and hydrolysis strategies for the release of fermentable sugars. Then, we optimized the growth of S. stipitis on the three media formulated from those biomasses, working on inoculum pre-adaptation, oxygen availability and supplementation of necessary nutrients to support the microorganism. Folate production, measured with a microbiological assay, reached 188.2 ± 24.86 μg/L on SBM, 130.6 ± 1.34 μg/L on SBP and 101.9 ± 6.62 μg/L on UGM. Here, we demonstrate the flexibility of S. stipitis in utilizing different residual biomasses as growth media. Moreover, we assessed the production of folate from waste, and to the best of our knowledge, we obtained the highest production of folate from residual biomasses ever reported, providing the first indications for the future development of this microbial production process.

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Characterisation of Breadfruit (Artocarpus altilis) Plants Growing on Lakoocha (A. lakoocha) Rootstocks.

Zhou, Y., & Underhill, S. J. (2022). Horticulturae, 8(10), 916.

Breadfruit (Artocarpus altilis) is a traditional fruit tree of 15–30 m tall in Oceania. The species is a staple crop for food security in the tropics. Tree loss from tropical windstorms, together with transition toward high-density planting has driven an interest in the dwarf phenotype of the species. Information on dwarfing rootstocks for breadfruit is currently limited. The aim of this study was to assess the performance of breadfruit growth with lakoocha (Artocarpus lakoocha) as rootstocks. We compared the phenotype of breadfruit trees on lakoocha rootstocks with those on self-graft and non-graft within 21 months after grafting. These led to the discovery of a rootstock-induced dwarf trait in breadfruit species. Breadfruit scions on lakoocha rootstocks displayed a reduction in tree height, stem thickness, and internode length, with fewer branches and leaves, resulting in about 32% of the standard height at the end of 21 months after grafting. These suggest lakoocha rootstocks have the potential to control breadfruit tree vigor. Non-structural carbohydrate analysis showed the composite trees exhibited lower hexose concentration in both scion stems and roots, but higher sucrose level in scion stems, and higher starch level in roots. The significance of these parameters in rootstock dwarfing is discussed.

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Croatian white grape variety Maraština: First taste of its indigenous mycobiota.

Milanović, V., Cardinali, F., Ferrocino, I., Boban, A., Franciosa, I., Kljusurić, J. G., Mucalo, A., Osimani, A., Aquilanti, L., Garofalo, C. & Budić-Leto, I. (2022). Food Research International, 162, 111917.

The indigenous vineyard mycobiota contribute both to wine quality and vineyard sanitary status. Wines made from same grape variety but from different geographical locations are appreciated for their diversity. Because no information on indigenous mycobiota of Croatian grapevines is available, the aim of the present study was to start filling this knowledge gap by characterizing the indigenous mycobiota of Maraština variety. The use of metataxonomic approach has enabled the identification of 25 different fungal genera present on Maraština grape berries collected from 11 vineyards located within the Croatian coastal winegrowing region of Dalmatia (Northern Dalmatia, Dalmatian hinterland, Central and Southern Dalmatia). The substantial regional and local scale differences in their distribution were observed. Overall, Aureobasidium was the dominant genus followed by Cladosporium and Metschnikowia. Botrytis and Plenodomus were associated with the vineyards located in Central and Southern Dalmatia, whereas Pichia was associated with Northern Dalmatia vineyards. The largest abundance of Buckleyzyma, Cladosporium, Eremothecium, Fusarium, Papiliotrema, and Rhodotorula was observed in Dalmatian hinterland. Moreover, data suggested that climate conditions and soil type partially influenced the distribution of fungal communities. The local-scale differences emerged also for the physicochemical characteristics of fresh musts. The high malic acid content supported the development of Metschnikowia, and inhibited Fusarium growth, whereas a positive correlation between Erysiphe and pH values was observed. Sporobolomyces and Cystobasidium were negatively associated with high glucose concentration. The revealing of Maraština indigenous mycobiota provided information on the members of fungal community negatively influencing the grapevine sanitary status as well as those which could be employed in disease biocontrol.

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Starch and protein analysis in buckwheat (Fagopyrum esculentum Moench) sprouts enriched with probiotic yeast.

Molska, M., Reguła, J., Zielińska-Dawidziak, M., Tomczak, A. & Świeca, M. (2022). LWT, 168, 113903.

The study aimed to investigate the effect of modification of Fagopyrum esculentum Moench sprouts by probiotic yeast strain on their nutritional value related to protein and starch. For this purpose, an attempt was also made to 1) evaluate the digestibility of protein and starch and 2) examine anti-nutritional factors that may affect the digestibility of the indicated macronutrients. Probiotic-rich sprouts were characterized by the highest content of total protein, free amino acids and peptides, resistant starch, and total free sugars. In addition, the amino acid profile of buckwheat sprouts changed compared to seeds. Modified buckwheat sprouts were characterized by a higher content of sulfur amino acids compared to seeds and control sprouts. The highest methionine content was found in sprouts rich in probiotics, 4.65 ± 0.29 mg/g 16gN. The results show also that the modification reduced protein and starch digestibility by 7.4% and 4.2%, respectively, compared to the seed. The digestibility may have been influenced by anti-nutritional compounds contained in the raw material, i.e. condensed tannins, phenols and α-amylase inhibitor. The presented research gives a new direction for the use of the raw material of buckwheat sprouts.

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Chemical composition and bioactivity of oilseed cake extracts obtained by subcritical and modified subcritical water.

Švarc-Gajić, J., Rodrigues, F., Moreira, M. M., Delerue-Matos, C., Morais, S., Dorosh, O., Silva, A. M., Bassani, A., Dzedik, V. & Spigno, G. (2022). Bioresources and Bioprocessing, 9(1), 1-14.

Recovery of bioactive compounds from biowaste is gaining more and more interest in circular economy models. The oilseed cakes are usually insufficiently exploited by most technologies since they represent valuable matrices abundant in proteins, minerals, and phytochemicals, but their use is mostly limited to feed ingredients, fertilizers or biofuel production. This study was thus focused on the exploration of new valorization pathways of oilseed cakes by subcritical water, representing a safe and economic alternative in the creation of value chains. Pumpkin, hemp, and flax seed cakes were treated with subcritical water in nitrogen and carbon-dioxide atmospheres, as well as in nitrogen atmosphere with the addition of acid catalyst. The degradation of carbohydrate fraction was studied by quantifying sugars and sugar degradation products in the obtained extracts. The extracts obtained under different conditions were further compared chemically with respect to total phenols and flavonoids, as well as to the content of individual phenolic compounds. Furthermore, the effects of subcritical water treatment conditions on antioxidant, antiradical and cytotoxic properties of thus obtained extracts were defined and discussed.

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Not-so-forbidden fruit: the potential conservation role of toxic Pimelea microcephala subsp. microcephala fruits for native arid zone birds.

Draper, J. T., Deo, P., Weinstein, P. & Simpson, B. S. (2022). Emu-Austral Ornithology, 122(2), 131-143.

Food resources in arid habitats are important for the survival of native fauna, especially where resources provide water or key nutrients during dry periods. However, food resource plants can be susceptible to grazing species or may not be suitable for revegetating arid areas. Pimelea microcephala subsp. microcephala (P.m.microcephala) is an Australian dioecious shrub bearing bright fruit that are likely to attract birds. The plant is also noted for its production of simplexin, a compound toxic to mammals. The aims of our study were 1) to assess the nutrient and simplexin content of P.m.microcephala fruits, and 2) to confirm the identity of native avian species that consume the fruits. With both pieces of information, we could then determine the conservation utility of P.m.microcephala. Combining chemical analysis of fruit nutrients with field observations, we found that ripe fruits contain 60.5% water, 2.8% sugar, and potent antioxidants, which would likely be of nutritional benefit to consuming frugivores. The fruits also contain high levels of the toxin simplexin, which comprised 3.6% of ripe fruits by weight. We identified eight bird species interacting with P.m.microcephala, with at least five of these consuming ripe fruits. Our study demonstrates the potential for P.m.microcephala to contribute to revegetation and provide a food resource for arid zone birds, whilst being protected from grazing by the presence of simplexin. Further studies are needed to establish the species’ absolute significance in terms of fruits as a source of water and nutrients to arid zone bird diets.

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Effect of 'loss of function' mutation in SER1 in wine yeast: fermentation outcomes in co-inoculation with non-Saccharomyces.

Lang, T. A., Walker, M. E., Boss, P. K. & Jiranek, V. (2022). OENO One, 56(2).

In wine fermentation, improved wine complexity and sensorial properties can arise from the use of non-Saccharomyces yeast. Generally less alcohol tolerant, such strains often do not finish fermentation, therefore requiring a second inoculation with the more robust Saccharomyces cerevisiae, usually added on Day 3. This sequential approach affords non-Saccharomyces time to make an impact before being overtaken by S. cerevisiae. However, two inoculations are inconvenient; therefore the identification of a slow growing S. cerevisiae strain that can be used in a single co-inoculation with the non-Saccharomyces yeast is highly attractive. In this study we investigated the use of the naturally occurring ‘loss of function’ SER1 variant, identified in a Sake yeast, for the purposes of carrying out co-inoculated wine fermentations. The SER1-232(G > C; G78R) change was introduced into the commonly used wine strain, EC1118, via CRISPR/Cas9 editing. In a chemically defined grape juice medium, the SER1(G78R) mutant grew and fermented more slowly and increased acetic acid, succinic acid and glycerol concentrations. Simultaneous inoculation with the slower-growing mutant with a Metschnikowia pulcherrima or Lachancea thermotolerans strain in sterile Sauvignon blanc juice resulted in differences in sensorial compounds, most likely derived from the presence of non-Saccharomyces yeasts. The EC1118 SER1 (G78R) mutant completed fermentation with M. pulcherrima, MP2, and in fact improved the viability of MP2 compared to when it was used as a monoculture. The SER1 (G78R) mutant also promoted both the growth of the SO2-sensitive L. thermotolerans strain, Viniflora® Concerto™, in a juice high in SO2 and its subsequent dominance during fermentation. In co-fermentations with wild-type EC1118, the Concerto™ population was substantially reduced with no significant changes in wine properties. This research adds to our understanding of the use of a novel slow-growing S. cerevisiae yeast in wine fermentations co-inoculated with non-Saccharomyces strains.

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Transcriptomic and proteomic data provide new insights into cold-treated potato tubers with T-and D-type cytoplasm.

Szajko, K., Sołtys-Kalina, D., Heidorn-Czarna, M., Smyda-Dajmund, P., Wasilewicz-Flis, I., Jańska, H. & Marczewski, W. (2022). Planta, 255(5), 1-12.

For the first time, we report differences in gene and protein expression in potato (Solanum tuberosum L.) tubers possessing the T- or D-type cytoplasm. Two F1 diploid reciprocal populations, referred to as T and D, were used. The pooling strategy was applied for detection of differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) in tubers consisting of extreme chip colour after cold storage. RNA and protein bulks were constructed from contrasting phenotypes. We recognized 48 and 15 DEGs for the T and D progenies, respectively. DEPs were identified in the amyloplast and mitochondrial fractions. In the T-type cytoplasm, only 2 amyloplast-associated and 5 mitochondria-associated DEPs were detected. Of 37 mitochondria-associated DEPs in the D-type cytoplasm, there were 36 downregulated DEPs in the dark chip colour bulks. These findings suggest that T- and D-type of cytoplasm might influence sugar accumulation in cold-stored potato tubers in different ways. We showed that the mt/nucDNA ratio was higher in D-possessing tubers after cold storage than in T progeny. For the D-type cytoplasm, the pt/nucDNA ratio was higher for tubers characterized by dark chip colour than for those with light chip colour. Our findings suggest that T- and D-type cytoplasm might influence sugar accumulation in cold-stored potato tubers in different ways.

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Impact of Lachancea thermotolerans on Chemical Composition and Sensory Profiles of Viognier Wines. Hranilovic, A., Albertin, W., Capone, D. L., Gallo, A., Grbin, P. R., Danner, L., Bastian, S., Masneuf-Pomarede, I., Coulon, J, Bely, M. & Jiranek, V. (2022). Journal of Fungi, 8(5), 474.

Impact of Lachancea thermotolerans on Chemical Composition and Sensory Profiles of Viognier Wines. Hranilovic, A., Albertin, W., Capone, D. L., Gallo, A., Grbin, P. R., Danner, L., Bastian, S., Masneuf-Pomarede, I., Coulon, J, Bely, M. & Jiranek, V. (2022). Journal of Fungi, 8(5), 474.

Viognier is a warm climate grape variety prone to loss of acidity and accumulation of excessive sugars. The yeast Lachancea thermotolerans can improve the stability and balance of such wines due to the partial conversion of sugars to lactic acid during alcoholic fermentation. This study compared the performance of five L. thermotolerans strains in co-inoculations and sequential inoculations with Saccharomyces cerevisiae in high sugar/pH Viognier fermentations. The results highlighted the dichotomy between the non-acidified and the bio-acidified L. thermotolerans treatments, with either comparable or up to 0.5 units lower pH relative to the S. cerevisiae control. Significant differences were detected in a range of flavour-active yeast volatile metabolites. The perceived acidity mirrored the modulations in wine pH/TA, as confirmed via “Rate-All-That-Apply” sensory analysis. Despite major variations in the volatile composition and acidity alike, the varietal aromatic expression (i.e., stone fruit aroma/flavour) remained conserved between the treatments.

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Associations among nutrient concentration, silage fermentation products, in vivo organic matter digestibility, rumen fermentation and in vitro methane yield in 78 grass silages.

Weiby, K. V., Krizsan, S. J., Eknæs, M., Schwarm, A., Whist, A. C., Schei, I., Steinshamn, H., Lund, P., Beauchemin, K. A. & Dønnem, I. (2022). Animal Feed Science and Technology, 285, 115249.

Grass-clover silage constitutes a large part of ruminant diets in Northern and Western Europe, but the impact of silage quality on methane (CH4) production is largely unknown. This study was conducted to identify the quality attributes of grass silage associated with variation in CH4 yield. We expected that silage nutrient concentrations and silage fermentation products would affect CH4 yield, and that these factors could be used to predict the methanogenic potential of the silages. Round bales (n = 78) of grass and grass-clover silage from 37 farms in Norway were sampled, incubated, and screened for in vitro CH4 yield, i.e. CH4 production expressed on the basis of incubated organic matter (CH4-OM) and digestible OM (CH4-dOM) using sheep. Concentration of indigestible neutral detergent fiber (iNDF) was quantified using the in situ technique. The data were subjected to correlation and principal component analyses. Stepwise multiple regression was used to model methanogenic potential of silages. Among all investigated silage composition variables, neutral detergent fiber (aNDFom) and water-soluble carbohydrate (WSC) concentrations obtained the greatest correlations to CH4-OM (r = −0.63 and r = 0.57, respectively, P < 0.001), while concentration of iNDF negatively correlated with CH4-OM (r = −0.48, P < 0.001). In vivo organic matter digestibility (OMD) and concentration of ammonia-N (NH3-N) in silages were also correlated to CH4-OM (r = 0.44 and r = −0.32, P < 0.001 and P < 0.01, respectively). The stepwise regression using CH4-OM as response variable included aNDFom, WSC, iNDF, silage propionic acid and pH in descending order. The stepwise regression using CH4-dOM as response variable included WSC, aNDFom and iNDF in descending order. Among in vitro rumen short chain fatty acids (SCFA), molar proportion of butyrate was the most prominent in increasing CH4-OM and CH4-dOM (r = 0.23 and r = 0.36, P < 0.05 and P < 0.01, respectively), while molar proportion of propionate was the most prominent SCFA in reducing CH4-OM and CH4-dOM (r = −0.23 and r = −0.26, respectively, P < 0.05). Regression models that account for silage quality attributes can be used to predict CH4 yield from silages with a coefficient of determination (R2) between 0.33 (CH4-dOM) and 0.65 (CH4-OM). In conclusion, concentration of WSC increased in vitro CH4-OM and CH4-dOM, while concentration of aNDFom and iNDF decreased CH4-OM and CH4-dOM in grass silages.

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Influence of sequential exogenous pretreatment and contact ultrasound-assisted air drying on the metabolic pathway of glucoraphanin in broccoli florets.

Liu, B., Tao, Y., Manickam, S., Li, D., Han, Y., Yu, Y. & Liu, D. (2022). Ultrasonics Sonochemistry, 84, 105977.

In this investigation, the combinations of exogenous pretreatment (melatonin or vitamin C) and contact ultrasound-assisted air drying were utilized to dry broccoli florets. To understand the influences of the studied dehydration methods on the conversion of glucoraphanin to bioactive sulforaphane in broccoli, various components (like glucoraphanin, sulforaphane, myrosinase, etc.) and factors (temperature and moisture) involved in the metabolism pathway were analyzed. The results showed that compared with direct air drying, the sequential exogenous pretreatment and contact ultrasound drying shortened the drying time by 19.0-22.7%. Meanwhile, contact sonication could promote the degradation of glucoraphanin. Both melatonin pretreatment and vitamin C pretreatment showed protective effects on the sulforaphane content and myrosinase activity during the subsequent drying process. At the end of drying, the sulforaphane content in samples dehydrated by the sequential melatonin (or vitamin C) pretreatment and ultrasound-intensified drying was 14.4% (or 26.5%) higher than only air-dried samples. The correlation analysis revealed that the exogenous pretreatment or ultrasound could affect the enzymatic degradation of glucoraphanin and the generation of sulforaphane through weakening the connections of sulforaphane-myrosinase, sulforaphane-VC, and VC-myrosinase. Overall, the reported results can enrich the biochemistry knowledge about the transformation of glucoraphanin to sulforaphane in cruciferous vegetables during drying, and the combined VC/melatonin pretreatment and ultrasound drying is conducive to protect bioactive sulforaphane in dehydrated broccoli.

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The C-Terminal Domain of Liquorilactobacillus nagelii Dextransucrase Mediates the Production of Larger Dextrans Compared to Liquorilactobacillus hordei.

Bechtner, J., Hassler, V., Wefers, D., Ehrmann, M. & Jakob, F. (2022). Gels, 8(3), 171.

Dextransucrases released by certain lactic acid bacteria form glucose polymers with predominantly α-1,6-linkages and may be exploited biotechnologically for the tailored production of polysaccharides with application potential. Despite releasing two closely related dextransucrases, previous studies showed that water kefir borne Liquorilactobacillus (L.) hordei TMW 1.1822 and L. nagelii TMW 1.1827 produce different amounts of polysaccharides with distinct particle sizes (molecular weight and radius of gyration) and molecular architectures. To investigate where these differences originate and thus to provide deeper insights into the functionally diverse nature of polysaccharide formation during water kefir fermentation, we constructed two variants of the L. nagelii dextransucrase-a full-length enzyme and a truncated variant, devoid of a C-terminal glucan-binding domain that reflects the domain architecture of the L. hordei dextransucrase-and applied them at various enzyme concentrations to form dextran over 24 h. The full-length enzyme exhibited a high activity, forming constant amounts of dextran until a four-fold dilution, whereas the truncated variant showed a gradual decrease in activity and dextran formation at an increasing dilution. The application of the full-length enzyme resulted in higher average particle sizes compared to the truncated variant. However, the dilution of the enzyme extracts also led to a slight increase in the average particle size in both enzymes. Neither the domain architecture nor the enzyme concentration had an impact on the structural architecture of the dextrans. The presented results thus suggest that the comparatively higher processivity of the L. nagelii dextransucrase is predominantly caused by the additional C-terminal glucan-binding domain, which is absent in the L. hordei dextransucrase. The average particle size may be influenced, to some extent, by the applied reaction conditions, whereas the structural architecture of the dextrans is most likely caused by differences in the amino acid sequence of the catalytic domain.

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Membrane-based Operations for the Fractionation of Polyphenols and Polysaccharides From Winery Sludges.

Mejia, J. A. A., Ricci, A., Figueiredo, A. S., Versari, A., Cassano, A., de Pinho, M. N. & Parpinello, G. P. (2022). Food and Bioprocess Technology, 15(4), 933-948.

The present work investigated the impact of ultrafiltration (UF) and nanofiltration (NF) membranes on the recovery and fractionation of polyphenolic compounds and polysaccharides from Sangiovese and Cabernet Sauvignon wine lees. A laboratory-made flat-sheet membrane in cellulose acetate (CA400-38) was used in the UF treatment of Sangiovese wine lees; three laboratory-made flat-sheet membranes in cellulose acetate (CA316, CA316-70, CA400-22) and a polyamide commercial membrane (NF90) were used in the NF treatment of Cabernet Sauvignon wine lees. All membranes were characterized in terms of hydraulic permeability and rejection toward references solutes; the performances of the membranes were measured in terms of productivity, fouling index, cleaning efficiency and retention toward target compounds. Experimental results indicated that all UF and NF membranes were effective in separating target compounds rejecting more than 92% of polysaccharides with polyphenols preferentially permeating through the membrane. The UF membrane rejected more than 40% of total polyphenols; rejections toward non-flavonoids and flavonoids were less than 25% and 12.5%, respectively. The laboratory-made NF membranes exhibited higher permeate flux values (of the order of 11–12 L/m2h) in comparison with the commercial NF membrane, despite the observed differences in the retention of specific solutes. Among the prepared membranes the CA316 showed a total rejection toward most part of non-flavonoids and flavonoids. The experimental results support the use of UF and NF processes in a sequential design to fractionate and refine phenolic compounds from winery sludge for the production of concentrated fractions with high antioxidant activities.

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Safety Information
Symbol : GHS05, GHS08
Signal Word : Danger
Hazard Statements : H314, H360
Precautionary Statements : P201, P202, P260, P264, P280
Safety Data Sheet
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