
20 mg
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Available for shipping
Content: | 20 mg |
Shipping Temperature: | Ambient |
Storage Temperature: | Ambient |
Physical Form: | Powder |
Stability: | > 2 years under recommended storage conditions |
CAS Number: | 79324-71-3 |
Synonyms: | Levanotriose, Fructofuranosyl-(2→6)-O-β-D-fructofuranosyl-(2→6)-O-β-D-Fructose |
Molecular Formula: | C18H32O16 |
Molecular Weight: | 504.44 |
Purity: | > 90% |
Substrate For (Enzyme): | endo-Levanase |
High purity Levantriose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Show list of oligosaccharides.
High-yield production and purification of prebiotic inulin-type fructooligosaccharides.
Wienberg, F., Hövels, M. & Deppenmeier, U. (2022). AMB Express, 12(1), 1-16.
Due to the health-promoting effects and functional properties of inulin-type fructooligosaccharides (I-FOS), the global market for I-FOS is constantly growing. Hence, there is a continuing demand for new, efficient biotechnological approaches for I-FOS production. In this work, crude inulosucrase InuGB-V3 from Lactobacillus gasseri DSM 20604 was used to synthesize I-FOS from sucrose. Supplementation with 1 mM CaCl2, a pH of 3.5-5.5, and an incubation temperature of 40°C were found to be optimal production parameters at which crude inulosucrase showed high conversion rates, low sucrose hydrolysis, and excellent stability over 4 days. The optimal process conditions were employed in cell-free bioconversion reactions. By elevating the substrate concentration from 570 to 800 g L−1, the I-FOS concentration and the synthesis of products with a low degree of polymerization (DP) could be increased, while sucrose hydrolysis was decreased. Bioconversion of 800 g L−1 sucrose for 20 h resulted in an I-FOS-rich syrup with an I-FOS concentration of 401 ± 7 g L−1 and an I-FOS purity of 53 ± 1% [w/w]. I-FOS with a DP of 3-11 were synthesized, with 1,1-kestotetraose (DP4) being the predominant transfructosylation product. The high-calorie sugars glucose, sucrose, and fructose were removed from the generated I-FOS-rich syrup using activated charcoal. Thus, 81 ± 5% of the initially applied I-FOS were recovered with a purity of 89 ± 1%.
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