Levantriose

Content: 20 mg
Shipping Temperature: Ambient
Storage Temperature: Ambient
Physical Form: Powder
Stability: > 6 months under recommended storage conditions
CAS Number: 79324-71-3
Synonyms: Levanotriose, Fructofuranosyl-(2→6)-O-β-D-fructofuranosyl-(2→6)-O-β-D-Fructose
Molecular Formula: C18H32O16
Molecular Weight: 504.44
Purity: > 90%
Substrate For (Enzyme): endo-Levanase

High purity Levantriose for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Show list of oligosaccharides.

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Certificate of Analysis
Safety Data Sheet
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Publication

High-yield production and purification of prebiotic inulin-type fructooligosaccharides.

Wienberg, F., Hövels, M. & Deppenmeier, U. (2022). AMB Express, 12(1), 1-16.

Due to the health-promoting effects and functional properties of inulin-type fructooligosaccharides (I-FOS), the global market for I-FOS is constantly growing. Hence, there is a continuing demand for new, efficient biotechnological approaches for I-FOS production. In this work, crude inulosucrase InuGB-V3 from Lactobacillus gasseri DSM 20604 was used to synthesize I-FOS from sucrose. Supplementation with 1 mM CaCl2, a pH of 3.5-5.5, and an incubation temperature of 40°C were found to be optimal production parameters at which crude inulosucrase showed high conversion rates, low sucrose hydrolysis, and excellent stability over 4 days. The optimal process conditions were employed in cell-free bioconversion reactions. By elevating the substrate concentration from 570 to 800 g L−1, the I-FOS concentration and the synthesis of products with a low degree of polymerization (DP) could be increased, while sucrose hydrolysis was decreased. Bioconversion of 800 g L−1 sucrose for 20 h resulted in an I-FOS-rich syrup with an I-FOS concentration of 401 ± 7 g L−1 and an I-FOS purity of 53 ± 1% [w/w]. I-FOS with a DP of 3-11 were synthesized, with 1,1-kestotetraose (DP4) being the predominant transfructosylation product. The high-calorie sugars glucose, sucrose, and fructose were removed from the generated I-FOS-rich syrup using activated charcoal. Thus, 81 ± 5% of the initially applied I-FOS were recovered with a purity of 89 ± 1%.

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Safety Data Sheet
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