|Content:||10 mL / 20,000 Units|
|Storage Temperature:||Below -10oC|
|Formulation:||In 50% (v/v) glycerol|
|Stability:||Minimum 1 year at < -10oC. Check vial for details.|
|EC Number:|| endo-Inulinase: 126.96.36.199, |
|CAS Number:|| 9001-57-4, |
|Synonyms:|| endo-inulinase: 1-beta-D-fructan fructanohydrolase |
exo-inulinase: fructan β-fructosidase
|Expression:||From Aspergillus sp.|
|Specificity:|| endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin. |
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
|Unit Definition:|| endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5. |
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC.
|Method recognition:||AOAC Method 997.08, AOAC Method 2000.11, AOAC Method 2016.06, GB Standard 5009.245-2016 and GB Standard 5009.255-2016|
High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For Fructan Determination (Liquid).
Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.
Components: exo-inulinase 2,000 U/ml (on kestose, at 40oC), endo-inulinase ˜ 100 U/ml (on fructan at 40oC), α-galactosidase < 0.08 U/ml (on polygalacturonic acid at 40oC), β-Glucanase < 0.04 U/ml (on β-glucan at 40oC) and pectinase < 0.07 U/ml (on pectin at 40oC).
Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).
Fructanase and sucrase (E-SUCR) are used in are used in the enzyme hydrolysis step of the AOAC method 2016.06 and the Chinese GB Standard 5009.255-2016 for the determination of fructan in foods.
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Validation of Methods
Multi-phytochemical determination of polar and non-polar garlic bioactive compounds in different food and nutraceutical preparations.
Ramirez, D. A., Altamirano, J. C. & Camargo, A. B. (2020). Food Chemistry, 337, 127648.
Amongst functional foods, garlic and its by-products stand out given their rich phytochemical profile. A comprehensive analytical approach becomes necessary to fully address garlic preparations health-promoting activities, considering the coexistence of several active ingredients from different chemical families. For this, we developed a multi-phytochemical protocol combining Ultrasound and Dispersive Liquid-Liquid Microextraction, coupled to Liquid Chromatography, for the determination of flavonols, organosulfur compounds, and inulin. Hydrophilic interaction chromatography showed an adequate resolution of flavonols and sugars in a shorter time. The protocol showed a suitable performance and acceptable quantitative yields for garlic powder, cooked garlic, black garlic, and liquid garlic flavouring samples. Additionally, the proposed methodology represented a useful tool to assess how the different garlic products related to functional properties, taking into account the various phytochemical families present in each sample. This is the first time a comprehensive and multi-phytochemical validated analysis of garlic preparations is proposed.Hide Abstract
Nemeth, C., Andersson, A. A. M., Andersson, R., Mangelsen, E., Sun, C. & Åman, P. (2014). Food and Nutrition Sciences, 5, 581-589.
Fructans are important in the survival of plants and also valuable for humans as potentially health promoting food ingredients. In this study fructan content and composition were determined in grains of 20 barley breeding lines and cultivars with a wide variation in chemical composition, morphology and country of origin, grown at one site in Chile. There was significant genotypic variation in grain fructan content ranging from 0.9% to 4.2% of grain dry weight. Fructan degree of polymerisation (DP) was analysed using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Changes in the distribution of different chain lengths and the pattern of structures of fructan were detected with increasing amount of fructan in the different barleys. A positive correlation was found between fructan content and the relative amount of long chain fructan (DP > 9) (r = 0.54, p = 0.021). Our results provide a basis for selecting promising barley lines and cultivars for further research on fructan in barley breeding with the aim to produce healthy food products.Hide Abstract
Revanna, R., Turnbull, M. H., Shaw, M. L., Wright, K. M., Butler, R. C., Jameson, P. E. & McCallum, J. A. (2013). Journal of the Science of Food and Agriculture, 93(10), 2470-2477.
Background: Non-structural carbohydrate (NSC; glucose, fructose, sucrose and fructan) composition of onions (Allium cepa L.) varies widely and is a key determinant of market usage. To analyse the physiology and genetics of onion carbohydrate metabolism and to enable selective breeding, an inexpensive, reliable and practicable sugar assay is required to phenotype large numbers of samples. Results: A rapid, reliable and cost-effective microplate-based assay was developed for NSC analysis in onions and used to characterise variation in tissue hexose, sucrose and fructan content in open-pollinated breeding populations and in mapping populations developed from a wide onion cross. Sucrose measured in microplates employing maltase as a hydrolytic enzyme was in agreement with HPLC-PAD results. The method revealed significant variation in bulb fructan content within open-pollinated ‘Pukekohe Longkeeper’ breeding populations over a threefold range. Very wide segregation from 80 to 600 g kg−1 in fructan content was observed in bulbs of F2 genetic mapping populations from the wide onion cross ‘Nasik Red × CUDH2150’. Conclusion: The microplate enzymatic assay is a reliable and practicable method for onion sugar analysis for genetics, breeding and food technology. Open-pollinated onion populations may harbour extensive within-population variability in carbohydrate content, which may be quantified and exploited using this method. The phenotypic data obtained from genetic mapping populations show that the method is well suited to detailed genetic and physiological analysis.Hide Abstract
Komatsu, T. R., Buriti, F. C. A., da Silva, R. C., Lobo, A. R., Colli, C., Gioielli, L. A. & Saad, S. M. I. (2013). LWT-Food Science and Technology, 50(2), 755-765.
Functional guava mousses were prepared with inulin (I) and whey protein concentrate (WPC), in different combinations, with the purpose of partially or totally substituting their milk fat (MF) content, using a simplex-centroid design. In order to verify the adequacy of mousses to comply the standards for the nutrient content and nutrient comparative claims, their composition and energy values were compared with the food legislation adopted currently in Brazil, the European Union (E.U.), and the United States (U.S.), besides the new proposal for the Brazilian standards. Most of the formulations, especially I, WPC, I + WPC, and MF + I + WPC, and except for MF + WPC, were able to fulfil the requisites for receiving the “low” (nutrient content) and “reduced” (comparative) claims for total and saturated fat. Also, products with inulin could achieve the requisites for the “high” claim for dietary fibre. Nonetheless, important differences between the legislations for achieving some claims were noted, especially when the serving portion was used as standard instead of 100 g. This would require some attention by regulatory authorities, once the possibility of manufacturers to reduce or to increase the products’ serving portions up to achieve a claim, misunderstanding the consumer, may exist.Hide Abstract
Rakha, A., Åman, P. & Andersson, R. (2010). Food Chemistry, 119(3), 859-867.
In this study, dietary fibre (DF) was characterised in rye products from a local supermarket. Soft breads generally had lower DF contents (8–18%) than had crisp breads (13–20%) due to high inclusion of wheat flour. For some products, the labelled DF values contained fructan, but others did not. However, for most products, the DF values analysed exceeded those declared. Arabinoxylan (AX) and fructan were generally the main DF components in the products, followed by cellulose and resistant starch, β-glucan, Klason lignin and arabinogalactan. In the soft breads, cellulose and resistant starch concentrations were relatively high, due to significant formation of resistant starch. During bread manufacturing, the molecular weight of β-glucan was highly degraded, while that of AX was more resistant. Extruded products had the highest β-glucan extractability and the extracted β-glucan retained its molecular weight most, which may be of nutritional significance. In rye milling fractions, about 50% of the fructan content analysed had a degree of polymerisation below 10, i.e. it comprised oligosaccharides. The crisp breads produced without yeast had the highest DF and fructan contents and the highest proportion of low-molecular weight fructan. These results indicate that, during bread-making, the low-molecular weight fraction of fructan was most available for degradation by yeast or by endogenous enzymes present in the ingredients.Hide Abstract
Rakha, A., Åman, P. & Andersson, R. (2011). International journal of molecular sciences, 12(5), 3381-3393.
Extractable dietary fiber (DF) plays an important role in nutrition. This study on porridge making with whole grain rye investigated the effect of rest time of flour slurries at room temperature before cooking and amount of flour and salt in the recipe on the content of DF components and molecular weight distribution of extractable fructan, mixed linkage (1→3)(1→4)-β-D-glucan (β-glucan) and arabinoxylan (AX) in the porridge. The content of total DF was increased (from about 20% to 23% of dry matter) during porridge making due to formation of insoluble resistant starch. A small but significant increase in the extractability of β-glucan (P = 0.016) and AX (P = 0.002) due to rest time was also noted. The molecular weight of extractable fructan and AX remained stable during porridge making. However, incubation of the rye flour slurries at increased temperature resulted in a significant decrease in extractable AX molecular weight. The molecular weight of extractable β-glucan decreased greatly during a rest time before cooking, most likely by the action of endogenous enzymes. The amount of salt and flour used in the recipe had small but significant effects on the molecular weight of β-glucan. These results show that whole grain rye porridge made without a rest time before cooking contains extractable DF components maintaining high molecular weights. High molecular weight is most likely of nutritional importance.Hide Abstract
Capriles, V. D., Soares, R. A. M., Pinto E Silva, M. E. M. & Arêas, J. A. G. (2009). International Journal of Food Science & Technology, 44(10), 1895-1901.
To develop a convenience healthy food snack the partially hydrogenated vegetable fat, used as the flavour fixative agent, was replaced by a non-fat-flavouring solution enriched with inulin and oligofructose. The effects of this replacement on chemical composition, in vitro rate of starch digestion and sensory acceptability were assessed. The new snack presented low-fat levels (0.1%) and around a sevenfold increase in dietary fibre (15.3% of dietary fibre, being 13.3% of fructans) when compared with the traditional ones. The enrichment with fructans reduced the predicted Glycaemic Index by 25%, thus indicating that this dietary fibre contributes effectively towards delaying the in vitro glycaemic response. Fructans-enriched snack presented overall acceptability score (6.6 ± 1.7) similar to the traditional one, flavoured with fatty fixative agent (7.4 ± 1.4). The healthy low-fat fibre-enriched snack produced presented the high sensory acceptability typical for this food product type.Hide Abstract