|Storage Temperature:||Below -10oC|
|Formulation:||Supplied as a freeze-dried powder|
|Stability:||Minimum 1 year at < -10oC. Check vial for details.|
endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase
endo-inulinase: Supplied at ~ 100 U/mL
exo-inulinase: Supplied at ~ 2,000 U/mL
|Expression:||Recombinant from Aspergillus niger|
endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC.
Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
Components: exo-Inulinase 20,000 U (on kestose at 40oC), endo-inulinase 1000 U (on fructan at 40oC).
Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).
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Validation of Methods