Fructanase Mixture
(Ultrapure, recombinant, powder)

Reference code: E-FRPDPU

20,000 Units

This product has been discontinued

Buy on Neogen.com

Share by email Ask a Scientist

Data Sheet

SDS

Presentations

Certificate of Analysis

Content:

20,000 Units

Shipping Temperature:

Ambient

Storage Temperature:

Below -10^oC

Formulation:

Supplied as a freeze-dried powder

Physical Form:

Powder

Stability:

> 1 year under recommended storage conditions

Enzyme Activity:

Fructanase

EC Number:

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80

CAZy Family:

GH32

CAS Number:

9001-57-4,
9025-67-6,
37288-56-5

Synonyms:

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase

Source:

Aspergillus niger

Concentration:

endo-inulinase: Supplied at ~ 1,000 U
exo-inulinase: Supplied at ~ 20,000 U

Expression:

Recombinant from Aspergillus niger

Specificity:

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

Unit Definition:

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40^oC.

Temperature Optima:

60^oC

pH Optima:

4.5

Validation of Methods

This product has been discontinued.

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

Components: exo-Inulinase 20,000 U (on kestose at 40^oC), endo-inulinase 1000 U (on fructan at 40^oC).

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

Here is our complete list of all our CAZyme products.