Fructanase Mixture
(Ultrapure, recombinant, liquid)

Reference code: E-FRLQPU

10 mL / 20,000 Units

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

10 mL / 20,000 Units

Shipping Temperature:

Ambient

Storage Temperature:

Below -10^oC

Formulation:

In 50% (v/v) glycerol plus 0.02% sodium azide

Physical Form:

Solution

Stability:

> 1 year under recommended storage conditions

Enzyme Activity:

Fructanase

EC Number:

endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80

CAZy Family:

GH32

CAS Number:

9001-57-4,
9025-67-6,
37288-56-5

Synonyms:

endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase

Source:

Aspergillus niger

Concentration:

endo-inulinase: Supplied at ~ 1,000 U
exo-inulinase: Supplied at ~ 20,000 U

Expression:

Recombinant from Aspergillus niger

Specificity:

endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.

Unit Definition:

endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40^oC.

Temperature Optima:

60^oC

pH Optima:

4.5

Application examples:

Applications in carbohydrate research and in the food industry.

Validation of Methods

This product has been discontinued.

High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and analytical testing applications.

For Fructan Determination.

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.

Components: exo-Inulinase (2,000 U/mL on kestose, at 40^oC) and endo-Inulinase (100 U/mL on inulin at 40^oC).

Note: This product is a mixture of two ultrapure, recombinant enzymes. Suitable for the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

Find out more of our available Carbohydrate Active enZYmes.