D-Fructose/D-Glucose Assay Kit (Liquid Ready) 

Reference code: K-FRGLQR
SKU: 700004283

1100 assays (microplate) / 1100 assays (auto-analyser)

Content: 1100 assays (microplate) / 1100 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 6 months under recommended storage conditions
Analyte: D-Fructose, D-Glucose
Assay Format: Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Increase
Linear Range: 0.4 to 20 µg of D-glucose plus D-fructose per assay
Limit of Detection: 133 mg/L
Reaction Time (min): ~ 13 min
Application examples: Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread, bakery products, candies, desserts, confectionery, ice-cream, fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals, paper and other materials (e.g. biological cultures, samples, etc.).
Method recognition: Methods based on this principle have been accepted by AOAC, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC

The D-Fructose/D-Glucose (Liquid Ready) test kit is a rapid, reliable and accurate method for the specific measurement and analysis of D-fructose and D-glucose in wine, beverages, foodstuffs and other materials. Supplied as a "ready to use" liquid stable formulation that is suitable for auto-analyser and microplate formats.

Find more of our monosaccharide and oligosaccharide assay kits.

Scheme-K-FRGLQR FRGLQR Megazyme

  • PVP incorporated to prevent tannin inhibition 
  • “Ready to use” liquid stable formulation 
  • Very competitive price (cost per test) 
  • All reagents stable for > 2 years 
  • Very rapid reaction (~ 13 min) 
  • Standard included 
  • Suitable for microplate and auto-analyser formats
Certificate of Analysis
Safety Data Sheet
FAQs Assay Protocol

Silica-Calcium-Alginate Hydrogels for the Co-Immobilization of Glucose Oxidase and Catalase to Reduce the Glucose in Grape Must.

Del-Bosque, D., Vila-Crespo, J., Ruipérez, V., Fernández-Fernández, E. & Rodríguez-Nogales, J. M. (2023). Gels, 9(4), 320.

Higher temperatures due to climate change are causing greater sugar production in grapes and more alcoholic wines. The use of glucose oxidase (GOX) and catalase (CAT) in grape must is a biotechnological green strategy to produce reduced-alcohol wines. GOX and CAT were effectively co-immobilized by sol-gel entrapment in silica-calcium-alginate hydrogel capsules. The optimal co-immobilization conditions were achieved at a concentration of the colloidal silica, sodium silicate and sodium alginate of 7.38%, 0.49% and 1.51%, respectively, at pH 6.57. The formation of a porous silica-calcium-alginate structure was confirmed by environmental scanning electron microscopy and the elemental analysis of the hydrogel by X-ray spectroscopy. The immobilized GOX showed a Michaelis-Menten kinetic, while the immobilized CAT fits better to an allosteric model. Immobilization also conferred superior GOX activity at low pH and temperature. The capsules showed a good operational stability, as they could be reused for at least 8 cycles. A substantial reduction of 26.3 g/L of glucose was achieved with encapsulated enzymes, which corresponds to a decrease in potential alcoholic strength of must of about 1.5% vol. These results show that co-immobilized GOX and CAT in silica-calcium-alginate hydrogels is a promising strategy to produce reduced-alcohol wines.

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Chemical Composition of Sour Beer Resulting from Supplementation the Fermentation Medium with Magnesium and Zinc Ions.

Ciosek, A., Fulara, K., Hrabia, O., Satora, P. & Poreda, A. (2020). Biomolecules, 10(12), 1599.

The bioavailability of minerals, such as zinc and magnesium, has a significant impact on the fermentation process. These metal ions are known to influence the growth and metabolic activity of yeast, but there are few reports on their effects on lactic acid bacteria (LAB) metabolism during sour brewing. This study aimed to evaluate the influence of magnesium and zinc ions on the metabolism of Lactobacillus brevis WLP672 during the fermentation of brewers’ wort. We carried out lactic acid fermentations using wort with different mineral compositions: without supplementation; supplemented with magnesium at 60 mg/L and 120 mg/L; and supplemented with zinc at 0.4 mg/L and 2 mg/L. The concentration of organic acids, pH of the wort and carbohydrate use was determined during fermentation, while aroma compounds, real extract and ethanol were measured after the mixed fermentation. The addition of magnesium ions resulted in the pH of the fermenting wort decreasing more quickly, an increase in the level of L-lactic acid (after 48 h of fermentation) and increased concentrations of some volatile compounds. While zinc supplementation had a negative impact on the L. brevis strain, resulting in a decrease in the L-lactic acid content and a higher pH in the beer. We conclude that zinc supplementation is not recommended in sour beer production using L. brevis WLP672.

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Synergistic effect of Aspergillus niger and Trichoderma reesei enzyme sets on the saccharification of wheat straw and sugarcane bagasse.

van den Brink, J., Maitan-Alfenas, G. P., Zou, G., Wang, C., Zhou, Z., Guimarães, V. M. & de Vries, R. P. (2014). Biotechnology Journal, 9(10), 1329-1338.

Plant-degrading enzymes can be produced by fungi on abundantly available low-cost plant biomass. However, enzymes sets after growth on complex substrates need to be better understood, especially with emphasis on differences between fungal species and the influence of inhibitory compounds in plant substrates, such as monosaccharides. In this study, Aspergillus niger and Trichoderma reesei were evaluated for the production of enzyme sets after growth on two “second generation” substrates: wheat straw (WS) and sugarcane bagasse (SCB). A. niger and T. reesei produced different sets of (hemi-)cellulolytic enzymes after growth on WS and SCB. This was reflected in an overall strong synergistic effect in releasing sugars during saccharification using A. niger and T. reesei enzyme sets. T. reesei produced less hydrolytic enzymes after growth on non-washed SCB. The sensitivity to non-washed plant substrates was not reduced by using CreA/Cre1 mutants of T. reesei and A. niger with a defective carbon catabolite repression. The importance of removing monosaccharides for producing enzymes was further underlined by the decrease in hydrolytic activities with increased glucose concentrations in WS media. This study showed the importance of removing monosaccharides from the enzyme production media and combining T. reesei and A. niger enzyme sets to improve plant biomass saccharification.

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The effect of temperature and film covers on the storage ability of Arbutus unedo L. fresh fruit.

Guerreiro, A. C., Gago, C. M. L., Miguel, M. G. C. & Antunes, M. D. C. (2013). Scientia Horticulturae, 159, 96-102.

The strawberry tree (Arbutus unedo L.) fruits are used in small quantities for fresh consumption despite their excellent flavor. The aim of this work was to evaluate the storage ability of strawberry tree fruits for fresh consumption. Harvested fruits were stored in polystyrene foam trays covered with two film types: linear low density polyethylene of 10 µm thickness (PPL) or polyethylene film perforated with holes of 10 mm diameter spaced 50 mm (PP). Through 15 days storage at 0, 3 and 6°C, fruits were analyzed for quality parameters. The strawberry tree fruits presented relevant qualitative properties and were appreciated by panelists. Color parameters (L*, h°, C*) had higher decrease at 3 and 6°C than at 0°C. The Brix almost did not change and firmness decreased mainly in the first 4 days storage. Ethanol and weight loss increased with temperature mostly from 3 to 6°C. Strawberry tree fruits are a good source of ascorbic acid, glucose, fructose, anthocyanins and antioxidant activity. Those properties are maintained through 15 days storage at 0°C followed by 3°C. Film covers showed no significant differences between them. The temperature of 0°C was the best for preservation of fruit quality through 15 days shelf-life.

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Safety Information
Symbol : GHS05, GHS08
Signal Word : Danger
Hazard Statements : H315, H318, H360
Precautionary Statements : P201, P202, P264, P280, P302+P352, P305+P351+P338
Safety Data Sheet
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