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|Stability:||> 10 years under recommended storage conditions|
|Monosaccharides (%):||Total fructose, sucrose, glucose content is < 0.1%|
|Main Chain Glycosidic Linkage:||β-2,1|
|Substrate For (Enzyme):||endo-Fructanase|
High purity inulin for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This compound can be used as an analytical standard or as a substrate for inulin degrading enzymes. This product contains inulin almost exclusively in GFn form (each oligosaccharide terminates in a glucose residue). Note that fructooligosaccharides (DP2-8) are also available from Megazyme (P-FOS28). The Fn form predominates in P-FOS28 (each oligosaccharide lacks a terminal glucose residue).
Moderation of inulin and polyphenolics contents in three cultivars of Helianthus tuberosus L. by potassium fertilization.
Michalska-Ciechanowska, A., Wojdyło, A., Bogucka, B. & Dubis, B. (2019). Agronomy, 9(12), 884.
Jerusalem artichoke, a widely consumed edible, is an excellent source of inulin and selected phytochemicals. However, the improvement of its chemical composition by potassium fertilization has not yet been studied. Thus, the aim of the study was to evaluate the effect of different potassium (K) fertilization levels (K2O 150 kg ha-1, 250 kg ha-1, 350 kg ha-1) on the content of inulin; profile and changes in polyphenolic compounds; and the antioxidant capacity, including on-line ABTS antioxidant profiles of freeze-dried tubers originated from Violette de Rennes, Topstar, and Waldspindel cultivars. Inulin content was highest in the early maturing cv. Topstar. The application of 350 kg ha-1 of K fertilizer rates during the growth of cv. Topstar increased the inulin content of tubers by 13.2% relative to the lowest K fertilizer rate of 150 kg ha-1. In cv. Violette de Rennes, inulin accumulation increased in response to the fertilizer rate of 250 kg ha-1. A further increase in K fertilizer rates had no effect on inulin content. The inulin content of cv. Waldspindel was not modified by any of the tested K fertilizer rates. Thus, the accumulation of the inulin was cultivar-dependent. In the cultivars analyzed, 11 polyphenolic compounds were identified and polyphenolic compound content was affected by the applied rate of potassium fertilizer, which was dependent on the cultivar. Chlorogenic acid was the predominant phenolic acid in all cultivars, and it accounted for around 66.4% of the identified polyphenolic compounds in cv. Violette de Rennes and for around 77% of polyphenolic compounds in cv. Waldspindel and Topstar.Hide Abstract
A rapid-throughput adaptable method for determining the monosaccharide composition of polysaccharides.
Amicucci, M. J., Galermo, A. G., Nandita, E., Vo, T. T. T., Liu, Y., Lee, M., Xu, G. & Lebrilla, C. B. & Lebrilla, C. B. (2019). International Journal of Mass Spectrometry, 438, 22-28.
Polysaccharides make up the largest non-water component of plant-based foods. Their ability to manipulate the gut microbiome and modulate the immune system has increased interest in the rapid elucidation of their structures. A necessary component for the structural characterization of polysaccharides is the determination of their monosaccharide composition. Current methods of monosaccharide analysis are not suitable for analyzing large sample-sets and are limited by their inability to analyze polysaccharides. We have developed a 96-well plate hydrolysis and derivatization procedure followed by a rapid and sensitive 10-min ultra-high performance liquid chromatography triple quadrupole mass spectrometry analysis capable of the absolute quantitation of 14 plant monosaccharides. Four polysaccharide standards, inulin, xyloglucan, arabinogalactan, and rhamnogalacturonan-I, which are commonly found in plants, were used to optimize and validate the method. The optimized conditions were applied to eight foods to show the method’s reproducibility and ability to analyze complicated and insoluble polysaccharide mixtures. This approach will allow researchers to obtain accurate and absolute quantitation of monosaccharides in the large sample-sets that are required for agricultural, food, clinical, and nutrition-based studies.Hide Abstract