β-Glucosidase (Phanerochaetechrysosporium)

Reference code: E-BGOSPC

460 Units at 40^oC;
~ 1,380 Units at 60^oC

This product has been discontinued

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Data Sheet

SDS

Certificate of Analysis

Content:

460 Units at 40^oC;
~ 1,380 Units at 60^oC

Shipping Temperature:

Ambient

Storage Temperature:

2-8^oC

Formulation:

In 3.2 M ammonium sulphate

Physical Form:

Suspension

Stability:

> 4 years at 4^oC

Enzyme Activity:

β-Glucosidase

EC Number:

3.2.1.21

CAZy Family:

GH3

CAS Number:

9001-22-3

Synonyms:

beta-glucosidase; beta-D-glucoside glucohydrolase

Source:

Phanerochaete chrysosporium

Molecular Weight:

84,500

Concentration:

Supplied at ~ 460 U/mL

Expression:

Recombinant from Phanerochaete chrysosporium

Specificity:

Hydrolysis of terminal non-reducing β-D-glucosidic bonds releasing β-D-glucose.

Specific Activity:

~ 100 U/mg (40^oC, pH 5.0 on p-nitrophenyl β-D-glucopyranoside);
~ 300 U/mg (60^oC, pH 5.0 on p-nitrophenyl β-D-glucopyranoside)

Unit Definition:

One Unit of β-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-glucopyranoside (5 mM) in sodium acetate buffer (100 mM), pH 5.0 at 40^oC.

Temperature Optima:

70^oC

pH Optima:

5

Application examples:

Applications established in diagnostics and research within the food and feed, carbohydrate and biofuels industries.

This product has been discontinued (read more).

High purity recombinant β-Glucosidase (Phanerochaete chrysosporium) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Full length enzyme containing the N-terminal cellulose-binding domain (CBM1) and a C-terminal catalytic domain (GH3).

Browse our complete Carbohydrate Active enZYme product listing.