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β-Glucan CFA Standard

Product code: P-BGCFA
€150.00

4 vials: contents of vials given on vial label.

Prices exclude VAT

Available for shipping

Content: 4 vials: contents of vials given on vial label
Shipping Temperature: Ambient
Storage Temperature: Ambient
Physical Form: Powder
Stability: > 10 years under recommended storage conditions
CAS Number: 9041-22-9
Source: Barley flour
Purity: > 98%
Monosaccharides (%): Glucose
Main Chain Glycosidic Linkage: β-1,4 and β-1,3
Substrate For (Enzyme): β-Glucanase/Lichenase

High purity β-Glucan CFA Standard (previously FIA Standard) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. 

Standard for use with CFA/Calcofluor β-glucan assay procedure. Standardised by the Megazyme enzymic β-glucan assay procedure.

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Documents
Certificate of Analysis
Safety Data Sheet
FAQs Booklet
Publications
Publication
Development of barley and yeast β-glucan aerogels for drug delivery by supercritical fluids.

Salgado, M., Santos, F., Rodríguez-Rojo, S., Reis, R. L., Duarte, A. R. C. & Cocero, M. J. (2017). Journal of CO2 Utilization, 22, 262-269.

Polysaccharide aerogels are a good alternative as carriers for drug delivery, since they allow high loading of the active compounds in matrices that are non-toxic, biocompatible and from a renewable feedstock. In this work, barley and yeast β-glucans aerogels were produced by gelation in aqueous solution, followed by solvent exchange and drying with supercritical CO2. First, viscoelastic properties and melting profile of the hydrogels were determined. Then, the obtained aerogels were analyzed regarding morphology, mechanical properties and behavior in physiological fluid. Both in the hydrogels and in the aerogels, big differences were observed between barley and yeast 7beta;-glucans due to their different chain structure and gelation behavior. Finally, impregnation of acetylsalicylic acid was performed at the same time as the drying of the alcogels with supercritical CO2. The release profile of the drug in PBS was analyzed in order to determine the mechanism governing the release from the β-glucan matrix.

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Publication
Cereal β-glucan quantification with calcofluor-application to cell culture supernatants.

Rieder, A., Knutsen, S. H., Ballance, S., Grimmer, S. & Airado-Rodríguez, D. (2012). Carbohydrate Polymers, 90(4), 1564-1572.

The specific binding of the fluorescent dye calcofluor to cereal β-glucan results in increased fluorescence intensity of the formed complex and is in use for the quantification of β-glucan above a critical molecular weight (MW) by flow injection analysis. In this study, this method was applied in a fast and easy batch mode. In order to emphasize the spectral information of the emission spectra of the calcofluor/β-glucan complexes, derivative signals were calculated. A linear relationship was found between the amplitude of the second derivative signals and the β-glucan concentration between 0.1 and 0.4 μg/mL. The low detection limit of this new method (0.045 μg/mL) enabled its use to study the transport of cereal β-glucans over differentiated Caco-2 cell monolayers. Additionally, the method was applied to quantify β-glucan in arabinoxylan samples, which correlated well with data by an enzyme based method.

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Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
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